ArticleGene therapy2000
Virus-like gene transfer into cells mediated by polyoma virus pseudocapsids.
Article in Gene therapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Microtubules in Polyomavirus Infection.Viruses · 2020Review
- Virus-Inspired Self-Assembled Nanofibers with Aggregation-Induced Emission for Highly Efficient and Visible Gene Delivery.ACS applied materials & interfaces · 2017Article
- Progresses towards safe and efficient gene therapy vectors.Oncotarget · 2015Review
- In vitro encapsulation of heterologous dsDNA into human parvovirus B19 virus-like particles.Molecular biotechnology · 2015Article
- Involvement of microtubular network and its motors in productive endocytic trafficking of mouse polyomavirus.PloS one · 2014Article
- Improvement of different vaccine delivery systems for cancer therapy.Molecular cancer · 2011Review
- Engineered biological entities for drug delivery and gene therapy protein nanoparticles.Progress in molecular biology and translational science · 2011Review
- Biological gene delivery vehicles: beyond viral vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Bovine papillomavirus type 1: from clathrin to caveolin.Journal of virology · 2008Article
- Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy.Biophysical journal · 2008Article
- Hamster polyomavirus-derived virus-like particles are able to transfer in vitro encapsidated plasmid DNA to mammalian cells.Virus genes · 2007Article
- Mouse polyomavirus enters early endosomes, requires their acidic pH for productive infection, and meets transferrin cargo in Rab11-positive endosomes.Journal of virology · 2006Article
- Role for centromeric heterochromatin and PML nuclear bodies in the cellular response to foreign DNA.Molecular and cellular biology · 2006Article
- Intracellular trafficking of nonviral vectors.Gene therapy · 2005Review
- Human papillomavirus type 31b infection of human keratinocytes does not require heparan sulfate.Journal of virology · 2005Article
- Uptake pathway of polyomavirus via ganglioside GD1a.Journal of virology · 2004Article
- Further evidence that papillomavirus capsids exist in two distinct conformations.Journal of virology · 2003Article
- Cell penetration and trafficking of polyomavirus.Journal of virology · 2003Article
- Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging: application to single virus-like particle entry into a cell.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Caveolae are involved in the trafficking of mouse polyomavirus virions and artificial VP1 pseudocapsids toward cell nuclei.Journal of virology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mouse polyoma virus-like particles (or pseudocapsids) are composed solely of recombinant viral coat protein. They can interact with DNA and transport it to cells, resulting in gene expression both in tissue culture and in mice. We demonstrate that DNA transfer in vitro depends on partial packaging of DNA within the virus-like capsid. Cell surface sialic acid residues and an intact microtubule network, required for viral infectivity, are also necessary for pseudocapsid-mediated gene expression from heterologous DNA. Thus, gene delivery in this system requires pathways utilised by polyoma virions, rather than proceeding via the 'nonspecific' endosomal route typical of nonviral systems such as liposomes or calcium phosphate precipitates. Despite the fact that all cells appear to internalise pseudocapsid/DNA complexes, only a proportion show productive gene delivery. Bulk internalisation of complexes is dependent on actin fibres, but not cell surface sialic acid or microtubules, indicating that a second transport pathway exists for pseudocapsids which is nonproductive for gene transfer. The model suggested by these data demonstrates the virus-like properties of the pseudocapsid system, and provides a basis for further development to produce a highly effective gene delivery vehicle. Gene Therapy (2000) 7, 2122-2131.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.