ArticleGene therapy2000
Plasmid DNA adsorbed onto cationic microparticles mediates target gene expression and antigen presentation by dendritic cells.
Article in Gene therapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Emerging Cationic Nanovaccines.Pharmaceutics · 2024Review
- Multifunctional Immunoadjuvants for Use in Minimalist Nucleic Acid Vaccines.Pharmaceutics · 2021Review
- Development of novel cationic microemulsion as parenteral adjuvant for influenza vaccine.Asian journal of pharmaceutical sciences · 2020Article
- Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants.Vaccines · 2020Article
- Assembly of erodible, DNA-containing thin films on the surfaces of polymer microparticles: toward a layer-by-layer approach to the delivery of DNA to antigen-presenting cells.Acta biomaterialia · 2009Article
- Polymeric Materials for Gene Delivery and DNA Vaccination.Advanced materials (Deerfield Beach, Fla.) · 2009Article
- Enhancement of poly(orthoester) microspheres for DNA vaccine delivery by blending with poly(ethylenimine).Biomaterials · 2008Article
- Dose-dependent protection against or exacerbation of disease by a polylactide glycolide microparticle-adsorbed, alphavirus-based measles virus DNA vaccine in rhesus macaques.Clinical and vaccine immunology : CVI · 2008Article
- Preparation, characterization, cytotoxicity and transfection efficiency of poly(DL-lactide-co-glycolide) and poly(DL-lactic acid) cationic nanoparticles for controlled delivery of plasmid DNA.International journal of pharmaceutics · 2007Article
- Enhanced and prolonged cross-presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles.Immunology · 2006Article
- Enhanced antigen presentation and immunostimulation of dendritic cells using acid-degradable cationic nanoparticles.Journal of controlled release : official journal of the Controlled Release Society · 2005Article
- Enhanced potency of plasmid DNA microparticle human immunodeficiency virus vaccines in rhesus macaques by using a priming-boosting regimen with recombinant proteins.Journal of virology · 2005Article
- CpG oligodeoxynucleotides adsorbed onto polylactide-co-glycolide microparticles improve the immunogenicity and protective activity of the licensed anthrax vaccine.Infection and immunity · 2005Article
- Novel approaches to vaccine delivery.Pharmaceutical research · 2004Review
- PLGA-PEI nanoparticles for gene delivery to pulmonary epithelium.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · 2004Article
- Composition and surface charge of DNA-loaded microparticles determine maturation and cytokine secretion in human dendritic cells.Pharmaceutical research · 2004Article
- Modification to the capsid of the adenovirus vector that enhances dendritic cell infection and transgene-specific cellular immune responses.Journal of virology · 2004Article
- Review
- Recent advances in the discovery and delivery of vaccine adjuvants.Nature reviews. Drug discovery · 2003Review
- The effect of CTAB concentration in cationic PLG microparticles on DNA adsorption and in vivo performance.Pharmaceutical research · 2003Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Dendritic cells (DC) play a key role in antigen presentation and activation of specific immunity. Much current research focuses on harnessing the potency of DC for vaccines, gene therapy, and cancer immunotherapy applications. However, DC are not readily transfected in vitro by traditional nonviral techniques. A novel DNA vaccine formulation was used to determine if DC are transfected in vitro. The formulation consists of plasmid DNA adsorbed on to cationic microparticles composed of the biodegradable polymer polylactide-co-glycolide (PLG) and the cationic surfactant, cetyltrimethylammonium bromide (CTAB). Using preparations of fluorescent-labeled plasmid DNA formulated on PLG-CTAB microparticles to study internalization by macrophages and dendritic cells in vitro and in vivo, we found that most, but not all, of the fluorescence was concentrated in endosomal compartments. Furthermore, uptake of plasmid DNA encoding HIV p55 gag adsorbed to PLG-CTAB microparticles by murine bone marrow-derived dendritic cells resulted in target gene expression, as detected by RT-PCR. The antigen was subsequently processed and presented, resulting in stimulation of an H-2kd-restricted, gag-specific T cell hybridoma. Activation of the hybridoma, detected by IL-2 production, was dose-dependent in the range of 0.1-20 microg DNA (10-2000 microg PLG) and was sustained up to 5 days after transfection. Thus, adsorption of plasmid DNA on PLG-CTAB microparticles provides a potentially useful nonviral approach for in vitro transfection of dendritic cells. Gene Therapy (2000) 7, 2105-2112.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.