ArticleNucleic acids research2001
Characterization and mutational analysis of yeast Dbp8p, a putative RNA helicase involved in ribosome biogenesis.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Annotation and functional prediction of RNA helicases inIMA fungus · 2025Review
- Identification of a novel nuclease activity in human DDX49 helicase.Royal Society open science · 2024Article
- RNA folding and functions of RNA helicases in ribosome biogenesis.RNA biology · 2022Review
- Cofactor-dependent specificity of a DEAD-box protein.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- DExD/H-box RNA helicases in ribosome biogenesis.RNA biology · 2013Review
- In vivo approaches to dissecting the function of RNA helicases in eukaryotic ribosome assembly.Methods in enzymology · 2012Article
- The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1.Nucleic acids research · 2011Article
- A rice DEAD-box protein, OsRH36, can complement an Arabidopsis atrh36 mutant, but cannot functionally replace its yeast homolog Dbp8p.Plant molecular biology · 2010Article
- Powering through ribosome assembly.RNA (New York, N.Y.) · 2009Review
- Towards the prediction of essential genes by integration of network topology, cellular localization and biological process information.BMC bioinformatics · 2009Article
- Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins.Biochemistry · 2008Article
- Mutation of the arginine finger in the active site of Escherichia coli DbpA abolishes ATPase and helicase activity and confers a dominant slow growth phenotype.Nucleic acids research · 2008Article
- Review
- Comprehensive mutational analysis of yeast DEXD/H box RNA helicases involved in large ribosomal subunit biogenesis.Molecular and cellular biology · 2006Article
- Comprehensive mutational analysis of yeast DEXD/H box RNA helicases required for small ribosomal subunit synthesis.Molecular and cellular biology · 2006Article
- Dead-box proteins: a family affair--active and passive players in RNP-remodeling.Nucleic acids research · 2006Review
- The nucleolar protein Esf2 interacts directly with the DExD/H box RNA helicase, Dbp8, to stimulate ATP hydrolysis.Nucleic acids research · 2006Article
- The splicing ATPase prp43p is a component of multiple preribosomal particles.Molecular and cellular biology · 2005Article
- Characterization of the ATPase and unwinding activities of the yeast DEAD-box protein Has1p and the analysis of the roles of the conserved motifs.Nucleic acids research · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA helicases of the DEAD box family are involved in almost all cellular processes involving RNA molecules. Here we describe functional characterization of the yeast RNA helicase Dbp8p (YHR169w). Our results show that Dbp8p is an essential nucleolar protein required for biogenesis of the small ribosomal subunit. In vivo depletion of Dbp8p resulted in a ribosomal subunit imbalance due to a deficit in 40S ribosomal subunits. Subsequent analyses of pre-rRNA processing by pulse-chase labeling, northern hybridization and primer extension revealed that the early steps of cleavage of the 35S precursor at sites A(1) and A(2) are inhibited and delayed at site A(0). Synthesis of 18S rRNA, the RNA moiety of the 40S subunit, is thereby blocked in the absence of Dbp8p. The involvement of Dbp8p as a bona fide RNA helicase in ribosome biogenesis is strongly supported by the loss of Dbp8p in vivo function obtained by site-directed mutagenesis of some conserved motifs carrying the enzymatic properties of the protein family.
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