ArticleNucleic acids research2001
Sp1 phosphorylation regulates inducible expression of platelet-derived growth factor B-chain gene via atypical protein kinase C-zeta.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein.Bioscience reports · 2020Article
- ΗΙF1α, EGR1 and SP1 co-regulate the erythropoietin receptor expression under hypoxia: an essential role in the growth of non-small cell lung cancer cells.Cell communication and signaling : CCS · 2019Article
- Article
- Pin1-mediated Sp1 phosphorylation by CDK1 increases Sp1 stability and decreases its DNA-binding activity during mitosis.Nucleic acids research · 2014Article
- A novel specificity protein 1 (SP1)-like gene regulating protein kinase C-1 (Pkc1)-dependent cell wall integrity and virulence factors in Cryptococcus neoformans.The Journal of biological chemistry · 2011Article
- Transforming growth factor-β-inducible early response gene 1 is a novel substrate for atypical protein kinase Cs.Cellular and molecular life sciences : CMLS · 2011Article
- Coordinated sumoylation and ubiquitination modulate EGF induced EGR1 expression and stability.PloS one · 2011Article
- Specificity protein 1 regulates fascin expression in esophageal squamous cell carcinoma as the result of the epidermal growth factor/extracellular signal-regulated kinase signaling pathway activation.Cellular and molecular life sciences : CMLS · 2010Article
- Platelet-Derived Growth Factor (PDGF)/PDGF Receptors (PDGFR) Axis as Target for Antitumor and Antiangiogenic Therapy.Pharmaceuticals (Basel, Switzerland) · 2010Review
- Transcriptional regulation of the human Na+/H+ exchanger NHE3 by serotonin in intestinal epithelial cells.Biochemical and biophysical research communications · 2009Article
- Sp1 phosphorylation and its regulation of gene transcription.Molecular and cellular biology · 2009Review
- Sp1-dependent activation of KLF4 is required for PDGF-BB-induced phenotypic modulation of smooth muscle.American journal of physiology. Heart and circulatory physiology · 2009Article
- Epidermal growth factor-dependent cyclooxygenase-2 induction in gliomas requires protein kinase C-delta.Oncogene · 2009Article
- Post-translational control of sp-family transcription factors.Current genomics · 2008Article
- Article
- Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated derepression [corrected] of transcription of the luteinizing hormone receptor gene.Molecular and cellular biology · 2006Article
- Interleukin-21 receptor gene induction in human T cells is mediated by T-cell receptor-induced Sp1 activity.Molecular and cellular biology · 2005Article
- An Sp1 response element in the Kaposi's sarcoma-associated herpesvirus open reading frame 50 promoter mediates lytic cycle induction by butyrate.Journal of virology · 2005Article
- Differential effects of shear stress and cyclic strain on Sp1 phosphorylation by protein kinase Czeta modulates membrane type 1-matrix metalloproteinase in endothelial cells.Endothelium : journal of endothelial cell researchArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Platelet-derived growth factor (PDGF) is a broadly expressed mitogenic and chemotactic factor with diverse roles in a number of physiologic and pathologic settings. The zinc finger transcription factors Sp1, Sp3 and Egr-1 bind to overlapping elements in the proximal PDGF B-chain promoter and activate transcription of this gene. The anthracycline nogalamycin has previously been reported to inhibit the capacity of Egr-1 to bind DNA in vitro. Here we used electrophoretic mobility shift assays to show that nogalamycin added to cells in culture did not alter the interaction of Egr-1 with the PDGF-B promoter. Instead, it enhanced the capacity of Sp1 to bind DNA. Nogalamycin increased PDGF-B mRNA expression at the level of transcription, which was abrogated by mutation of the Sp1 binding site in the PDGF-B promoter or overexpression of mutant Sp1. Rather than increasing total levels of Sp1, nogalamycin altered the phosphorylation state of the transcription factor. Overexpression of dominant-negative PKC-zeta blocked nogalamycin-inducible Sp1 phosphorylation and PDGF-B promoter-dependent expression. Nogalamycin stimulated the phosphorylation of PKC-zeta (on residue Thr(410)). These findings demonstrate for the first time that PKC-zeta and Sp1 phosphorylation mediate the inducible expression of this growth factor.
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