Evidence map›Paper›PMID 11222750›Full record

ReviewNucleic acids research2001

Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.

T Pederson

Open access · bronzeAbstract readReview
In one paragraph

Review in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 26 citations in OpenAlex.

  1. The nucleus introduced.Cold Spring Harbor perspectives in biology · 2011
    Review
  2. The nucleolus.Cold Spring Harbor perspectives in biology · 2011
    Review
  3. Article
  4. Article
  5. Single mRNA tracking in live cells.Methods in enzymology · 2010
    Article
  6. Article
  7. Imaging of single mRNAs in the cytoplasm of living cells.Progress in molecular and subcellular biology · 2004
    Review
  8. Article
  9. Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis.Proceedings of the National Academy of Sciences of the United States of America · 2003
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

T PedersonDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 377 Plantation Street, Worcester, MA 01605, USA. thoru.pederson@umassmed.edu
University of Massachusetts Chan Medical School · US

Funding

RNA PROCESSING AND RIBONUCLEOPROTEINR01GM021595 · NIGMS · WORCESTER FOUNDATION FOR BIOMEDICAL RES · PI PEDERSON, THORU · 1985 to 2001
$886k
NIGMS NIH HHS GM-21595
6 · The paper itself

Abstract

The advent of jellyfish green fluorescent protein and its spectral variants, together with promising new fluorescent proteins from other classes of the Cnidarian phylum (coral and anemones), has greatly enhanced and promises to further boost the detection and localization of proteins in cell biology. It has been less widely appreciated that highly sensitive methods have also recently been developed for detecting the movement and localization in living cells of the very molecules that precede proteins in the gene expression pathway, i.e. RNAs. These approaches include the microinjection of fluorescent RNAs into living cells, the in vivo hybridization of fluorescent oligonucleotides to endogenous RNAs and the expression in cells of fluorescent RNA-binding proteins. This new field of 'fluorescent RNA cytochemistry' is summarized in this article, with emphasis on the biological insights it has already provided. These new techniques are likely to soon collaborate with other emerging approaches to advance the investigation of RNA birth, RNA-protein assembly and ribonucleoprotein particle transport in systems such as oocytes, embryos, neurons and other somatic cells, and may even permit the observation of viral replication and transcription pathways as they proceed in living cells, ushering in a new era of nucleic acids research in vivo.

Indexed as

AnimalsBiological TransportCellsFluorescent DyesHistocytochemistryHumansMicroinjectionsRNATranscription, GeneticFluorescent DyesRNA

Identifiers

PMID11222750
PMCPMC29735
OpenAlexW2134788865

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.