ArticleJournal of bacteriology2001
Identification of an Actinobacillus pleuropneumoniae consensus promoter structure.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Some novel features of strong promoters discovered in Cytophaga hutchinsonii.Applied microbiology and biotechnology · 2022Article
- Mutational analysis of the ompA promoter from Flavobacterium johnsoniae.Journal of bacteriology · 2007Article
- Distribution of tetracycline resistance genes in Actinobacillus pleuropneumoniae isolates from Spain.Antimicrobial agents and chemotherapy · 2006Article
- Promoters from a cold-adapted bacterium: definition of a consensus motif and molecular characterization of UP regulative elements.Extremophiles : life under extreme conditions · 2004Article
- Host cell contact-induced transcription of the type IV fimbria gene cluster of Actinobacillus pleuropneumoniae.Infection and immunity · 2004Article
- ohr, Encoding an organic hydroperoxide reductase, is an in vivo-induced gene in Actinobacillus pleuropneumoniae.Infection and immunity · 2002Article
- Strong minor groove base conservation in sequence logos implies DNA distortion or base flipping during replication and transcription initiation.Nucleic acids research · 2001Article
Corrections and comments
- Retraction · 2003-08-01Concerns/Issues about Data · Concerns/Issues about Results and/or Conclusions · Results Not Reproducible ·
- Retracted
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actinobacillus pleuropneumoniae promoter-containing clones were isolated from a genomic DNA library constructed in our lVET promoter trap vector pTF86. The promoter-containing clones were identified by their ability to drive expression of the promoterless luxAB genes of Vibrio harveyi. The degree of expression was quantifiable, and only high-expression or "hot" promoters were used for this study. Nine clones were sequenced, and their transcriptional start sites were determined by primer extension. The sequences upstream of the start site were aligned, and a consensus promoter structure for A. pleuropneumoniae was identified. The consensus promoter sequence for A. pleuropneumoniae was found to be TATAAT and TTG/AAA, centered approximately 10 and 35 bp upstream of the transcriptional start site, respectively. A comparison of the A. pleuropneumoniae consensus with other prokaryotic consensus promoters showed that the A. pleuropneumoniae consensus promoter is similar to that found in other eubacteria in terms of sequence, with an identical -10 element and a similar but truncated -35 element. However, the A. pleuropneumoniae consensus promoter is unique in the spacing between the -10 and -35 elements. The promoter spacing was analyzed by site-directed mutagenesis, which demonstrated that optimal spacing for an A. pleuropneumoniae promoter is shorter than the spacing identified for Escherichia coli and Bacillus subtilis promoters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.