ArticleBiophysical journal2001
Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Nanowaveguide-illuminated fluorescence correlation spectroscopy for single molecule studies.AIP advances · 2021Article
- Toward Absolute Molecular Numbers in DNA-PAINT.Nano letters · 2019Article
- Determination of multivalent protein-ligand binding kinetics by second-harmonic correlation spectroscopy.Analytical chemistry · 2014Article
- Mathematical modelling and computational study of two-dimensional and three-dimensional dynamics of receptor-ligand interactions in signalling response mechanisms.Journal of mathematical biology · 2014Article
- Engineering lipid bilayer membranes for protein studies.International journal of molecular sciences · 2013Review
- Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations.The journal of physical chemistry. B · 2011Article
- Photonic methods to enhance fluorescence correlation spectroscopy and single molecule fluorescence detection.International journal of molecular sciences · 2010Review
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Surface modified single molecules free-diffusion evidenced by fluorescence correlation spectroscopy.Journal of fluorescence · 2008Article
- Total internal reflection fluorescence correlation spectroscopy: effects of lateral diffusion and surface-generated fluorescence.Biophysical journal · 2008Article
- FCS imaging--a way to look at cellular processes.Biophysical journal · 2008Article
- Supercritical angle fluorescence correlation spectroscopy.Biophysical journal · 2008Article
- GM1 clustering inhibits cholera toxin binding in supported phospholipid membranes.Journal of the American Chemical Society · 2007Article
- Lateral mobility of membrane-binding proteins in living cells measured by total internal reflection fluorescence correlation spectroscopy.Biophysical journal · 2006Article
- Fluorescence correlation spectroscopy simulations of photophysical phenomena and molecular interactions: a molecular dynamics/monte carlo approach.The journal of physical chemistry. B · 2006Article
- lambda-Repressor oligomerization kinetics at high concentrations using fluorescence correlation spectroscopy in zero-mode waveguides.Biophysical journal · 2005Article
- High count rates with total internal reflection fluorescence correlation spectroscopy.Biophysical journal · 2005Article
- Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS).Biophysical journal · 2004Article
- Total internal reflection with fluorescence correlation spectroscopy: nonfluorescent competitors.Biophysical journal · 2004Article
- Ligand-receptor kinetics measured by total internal reflection with fluorescence correlation spectroscopy.Biophysical journal · 2003Article
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2 authors.
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Abstract
Total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is a method for measuring the surface association/dissociation rates and absolute densities of fluorescent molecules at the interface of solution and a planar substrate. This method can also report the apparent diffusion coefficient and absolute concentration of fluorescent molecules very close to the surface. An expression for the fluorescence fluctuation autocorrelation function in the absence of contributions from diffusion through the evanescent wave, in solution, has been published previously (N. L. Thompson, T. P. Burghardt, and D. Axelrod. 1981, Biophys. J. 33:435-454). This work describes the nature of the TIR-FCS autocorrelation function when both surface association/dissociation kinetics and diffusion through the evanescent wave contribute to the fluorescence fluctuations. The fluorescence fluctuation autocorrelation function depends in general on the kinetic association and dissociation rate constants, the surface site density, the concentration of fluorescent molecules in solution, the solution diffusion coefficient, and the depth of the evanescent field. Both general and approximate expressions are presented.
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