ArticleBiophysical journal2001
Optical and acoustical dynamics of microbubble contrast agents inside neutrophils.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 135 citations in OpenAlex.
- Imaging of macrophage accumulation in solid tumors with ultrasound.Nature communications · 2025Article
- Twinkling Peptide Nanoemulsions Enable Precision Ultrasound Detection of Atherosclerotic Plaques.Advanced functional materials · 2025Article
- Dynamics of crevice microbubbles that cause the twinkling artifact.Ultrasonics sonochemistry · 2024Article
- Real-Time, In Situ Imaging of Macrophages via Phase-Change Peptide Nanoemulsions.Small (Weinheim an der Bergstrasse, Germany) · 2023Article
- Intracellular vesicle entrapment of nanobubble ultrasound contrast agents targeted to PSMA promotes prolonged enhancement and stabilityNanotheranostics · 2022Article
- Ultrasound molecular imaging: insights into cardiovascular pathology.Journal of echocardiography · 2020Review
- Leveraging the Imaging Transmit Pulse to Manipulate Phase-Change Nanodroplets for Contrast-Enhanced Ultrasound.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2019Article
- Administration of Oxygen Ultra-Fine Bubbles Improves Nerve Dysfunction in a Rat Sciatic Nerve Crush Injury Model.International journal of molecular sciences · 2018Article
- Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2017Article
- In Vivo Transfection and Detection of Gene Expression of Stem Cells Preloaded with DNA-carrying Microbubbles.Radiology · 2015Article
- Echogenic perfluorohexane-loaded macrophages adhere in vivo to activated vascular endothelium in mice, an explorative study.Cardiovascular ultrasound · 2015Article
- Neural progenitor cells labeling with microbubble contrast agent for ultrasound imaging in vivo.Biomaterials · 2013Article
- Microbubble-mediated ultrasound therapy: a review of its potential in cancer treatment.Drug design, development and therapy · 2013Review
- Fluorescent microscope system to monitor real-time interactions between focused ultrasound, echogenic drug delivery vehicles, and live cell membranes.Ultrasonics · 2013Article
- Ultrasound imaging of oxidative stress in vivo with chemically-generated gas microbubbles.Annals of biomedical engineering · 2012Article
- Model for bubble pulsation in liquid between parallel viscoelastic layers.The Journal of the Acoustical Society of America · 2012Article
- Phase-change contrast agents for imaging and therapy.Current pharmaceutical design · 2012Review
- Future applications of contrast ultrasound.Journal of cardiovascular ultrasound · 2011Article
- Targeted renal therapies through microbubbles and ultrasound.Advanced drug delivery reviews · 2010Review
- Imaging myocardial metabolic remodeling.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2010Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Acoustically active microbubbles are used for contrast-enhanced ultrasound assessment of organ perfusion. In regions of inflammation, contrast agents are captured and phagocytosed by activated neutrophils adherent to the venular wall. Using direct optical observation with a high-speed camera and acoustical interrogation of individual bubbles and cells, we assessed the physical and acoustical responses of both phagocytosed and free microbubbles. Optical analysis of bubble radial oscillations during insonation demonstrated that phagocytosed microbubbles experience viscous damping within the cytoplasm and yet remain acoustically active and capable of large volumetric oscillations during an acoustic pulse. Fitting a modified version of the Rayleigh-Plesset equation that describes mechanical properties of thin shells to optical radius-time data of oscillating bubbles provided estimates of the apparent viscosity of the intracellular medium. Phagocytosed microbubbles experienced a viscous damping approximately sevenfold greater than free microbubbles. Acoustical comparison between free and phagocytosed microbubbles indicated that phagocytosed microbubbles produce an echo with a higher mean frequency than free microbubbles in response to a rarefaction-first single-cycle pulse. Moreover, this frequency increase is predicted using the modified Rayleigh-Plesset equation. We conclude that contrast-enhanced ultrasound can detect distinct acoustic signals from microbubbles inside of neutrophils and may provide a unique tool to identify activated neutrophils at sites of inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.