ArticleBiophysical journal2001
Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 68 citations in OpenAlex.
- Optical nanoscopy of spatiotemporal metal stripping cooperativity at single-ion and subparticle resolution.Nature materials · 2026Article
- A Fluorometric Method for Zinc Estimation: Applications in the Estimation of Plasma Zinc and in Assessing Zinc Bioaccessibility from Rice.Biological trace element research · 2025Article
- Article
- Steric and Stereochemical Modulation in Pyridyl- and Quinolyl-Containing Ligands.Molecules (Basel, Switzerland) · 2016Review
- Zinc-permeable ion channels: effects on intracellular zinc dynamics and potential physiological/pathophysiological significance.Current medicinal chemistry · 2015Review
- Selectivity and specificity of small molecule fluorescent dyes/probes used for the detection of Zn2+ and Ca2+ in cells.Metallomics : integrated biometal science · 2014Article
- Evidence for an extracellular zinc-veneer in rodent brains from experiments with Zn-ionophores and ZnT3 knockouts.ACS chemical neuroscience · 2012Article
- Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution.Inorganic chemistry · 2011Article
- Cytosolic zinc release and clearance in hippocampal neurons exposed to glutamate--the role of pH and sodium.Journal of neurochemistry · 2011Article
- Article
- The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green.Analytical biochemistry · 2009Article
- Effect of TPEN on the calcium release of cultured C2C12 mouse myotubes.Journal of muscle research and cell motility · 2007Article
- Neuronal zinc exchange with the blood vessel wall promotes cerebral amyloid angiopathy in an animal model of Alzheimer's disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Detecting and minimizing zinc contamination in physiological solutions.BMC physiology · 2004Article
- The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.Molecular biology of the cell · 2004Article
- Evidence for chelatable zinc in the extracellular space of the hippocampus, but little evidence for synaptic release of Zn.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
High concentrations of free Zn2+ ions are found in certain glutamatergic synaptic vesicles in the mammalian brain. These terminals can be visualized histochemically with quinoline sulfonamide compounds that form fluorescent complexes with Zn2+. The present study was undertaken to examine the interaction of the water-soluble quinoline sulfonamide probe, Zinquin (2-methyl-8-(toluene-p-sulfonamido)-6-quinolyloxyacetic acid) with the complex heterogeneous cellular environment. Experiments on rat hippocampal and neocortical slices gave indications that Zinquin in its free acid form was able to diffuse across the plasma and synaptic vesicle membranes. Further experiments were undertaken on unilamellar liposomes to study the interaction of Zinquin and its metal complexes in membranes. These experiments confirmed that Zinquin is able to diffuse across lipid bilayers. Steady-state and time-resolved fluorimetric studies showed that Zinquin in aqueous solution mainly forms a 1:2 (metal:ligand) complex with small amounts of a 1:1 complex. Formation of the 1:1 complex was favored by the presence of lipid, suggesting that it partitions into membranes. Evidence is presented that Zinquin can act as a Zn(2+)-ionophore, exchanging Zn2+ for two protons. The presence of a pH gradient across vesicles traps the Zn(2+)-probe complex within the vesicles. Zinquin is useful as a qualitative probe for detecting the presence of vesicular Zn2+; however, its tendency to partition into membranes and to serve as an ionophore should be borne in mind.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.