ArticleBiophysical journal2001
Alternatively spliced alpha(1G) (Ca(V)3.1) intracellular loops promote specific T-type Ca(2+) channel gating properties.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- The four voltage-sensing domains of T-type calcium channels activate near the resting membrane potential.Nature communications · 2026Article
- Electrophysiological characterization of a CaPflugers Archiv : European journal of physiology · 2023Article
- Structure, gating, and pharmacology of human CaNature communications · 2022Article
- Small Molecules as Modulators of Voltage-Gated Calcium Channels in Neurological Disorders: State of the Art and Perspectives.Molecules (Basel, Switzerland) · 2022Review
- Modeling temperature- and Cav3 subtype-dependent alterations in T-type calcium channel mediated burst firing.Molecular brain · 2021Article
- Functional Microstructure of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Novel MissenseInternational journal of molecular sciences · 2020Article
- Neuronal Cav3 channelopathies: recent progress and perspectives.Pflugers Archiv : European journal of physiology · 2020Review
- T-type, but not L-type, voltage-gated calcium channels are dispensable for lymphatic pacemaking and spontaneous contractions.Scientific reports · 2020Article
- Genetic T-type calcium channelopathies.Journal of medical genetics · 2020Review
- The Cacna1h mutation in the GAERS model of absence epilepsy enhances T-type CaScientific reports · 2017Article
- Evolutionary insights into T-type CaThe Journal of general physiology · 2017Article
- T-type calcium channels in synaptic plasticity.Channels (Austin, Tex.) · 2017Review
- Age-dependent impact of CaThe Journal of physiology · 2016Article
- Regulation of the T-type Ca(2+) channel Cav3.2 by hydrogen sulfide: emerging controversies concerning the role of H2 S in nociception.The Journal of physiology · 2016Review
- Sodium channel diversity in the vestibular ganglion: NaV1.5, NaV1.8, and tetrodotoxin-sensitive currents.Journal of neurophysiology · 2016Article
- Targeting voltage-gated calcium channels in neurological and psychiatric diseases.Nature reviews. Drug discovery · 2016Review
- The Physiology, Pathology, and Pharmacology of Voltage-Gated Calcium Channels and Their Future Therapeutic Potential.Pharmacological reviews · 2015Review
- Calcium Channel CaVα₁ Splice Isoforms - Tissue Specificity and Drug Action.Current molecular pharmacology · 2015Review
- Cav3 T-type channels: regulators for gating, membrane expression, and cation selectivity.Pflugers Archiv : European journal of physiology · 2014Review
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
At least three genes encode T-type calcium channel alpha(1) subunits, and identification of cDNA transcripts provided evidence that molecular diversity of these channels can be further enhanced by alternative splicing mechanisms, especially for the alpha(1G) subunit (Ca(V)3.1). Using whole-cell patch-clamp procedures, we have investigated the electrophysiological properties of five isoforms of the human alpha(1G) subunit that display a distinct III-IV linker, namely, alpha(1G-a), alpha(1G-b), and alpha(1G-bc), as well as a distinct II-III linker, namely, alpha(1G-ae), alpha(1G-be), as expressed in HEK-293 cells. We report that insertion e within the II-III linker specifically modulates inactivation, steady-state kinetics, and modestly recovery from inactivation, whereas alternative splicing within the III-IV linker affects preferentially kinetics and voltage dependence of activation, as well as deactivation and inactivation. By using voltage-clamp protocols mimicking neuronal activities, such as cerebellar train of action potentials and thalamic low-threshold spike, we describe that inactivation properties of alpha(1G-a) and alpha(1G-ae) isoforms can support channel behaviors reminiscent to those described in native neurons. Altogether, these data demonstrate that expression of distinct variants for the T-type alpha(1G) subunit can account for specific low-voltage-activated currents observed in neuronal tissues.
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