ArticleBiophysical journal2001
Dynamic contact forces on leukocyte microvilli and their penetration of the endothelial glycocalyx.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 113 citations in OpenAlex.
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- Mechanosurveillance: Tiptoeing T Cells.Frontiers in immunology · 2022Review
- CCR7 signalosomes are preassembled on tips of lymphocyte microvilli in proximity to LFA-1.Biophysical journal · 2021Article
- Endothelial Glycocalyx Layer Properties and Its Ability to Limit Leukocyte Adhesion.Biophysical journal · 2020Article
- A Lifetime Achievement in Bioengineering: Professor Shu Chien.Annals of biomedical engineering · 2019Article
- Synthetic Semiflexible and Bioactive Brushes.Biomacromolecules · 2019Article
- Endothelial Nanomechanics in the Context of Endothelial (Dys)function and Inflammation.Antioxidants & redox signaling · 2019Review
- Inhibition of inflammation induced shedding of the endothelial glycocalyx with low molecular weight heparin.Microvascular research · 2017Article
- Low-volume resuscitation with normal saline is associated with microvascular endothelial dysfunction after hemorrhage in rats, compared to colloids and balanced crystalloids.Critical care (London, England) · 2017Article
- Nanomechanics of the endothelial glycocalyx contribute to NaScientific reports · 2017Article
- Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis.Nature communications · 2017Article
- Differential structural remodelling of heparan sulfate by chemokines: the role of chemokine oligomerization.Open biology · 2017Article
- Flow-Enhanced Stability of Rolling Adhesion through E-Selectin.Biophysical journal · 2016Article
- Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall.Cellular and molecular bioengineering · 2016Article
- Role of the endothelial surface layer in neutrophil recruitment.Journal of leukocyte biology · 2015Review
- Article
- TRAIL-coated leukocytes that kill cancer cells in the circulation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We develop a theoretical model to examine the combined effect of gravity and microvillus length heterogeneity on tip contact force (F(m)(z)) during free rolling in vitro, including the initiation of L-, P-, and E-selectin tethers and the threshold behavior at low shear. F (m)(z) grows nonlinearly with shear. At shear stress of 1 dyn/cm(2), F(m)(z) is one to two orders of magnitude greater than the 0.1 pN force for gravitational settling without flow. At shear stresses > 0.2 dyn/cm(2) only the longest microvilli contact the substrate; hence at the shear threshold (0.4 dyn/cm(2) for L-selectin), only 5% of microvilli can initiate tethering interaction. The characteristic time for tip contact is surprisingly short, typically 0.1-1 ms. This model is then applied in vivo to explore the free-rolling interaction of leukocyte microvilli with endothelial glycocalyx and the necessary conditions for glycocalyx penetration to initiate cell rolling. The model predicts that for arteriolar capillaries even the longest microvilli cannot initiate rolling, except in regions of low shear or flow reversal. In postcapillary venules, where shear stress is approximately 2 dyn/cm(2), tethering interactions are highly likely, provided that there are some relatively long microvilli. Once tethering is initiated, rolling tends to ensue because F(m)(z) and contact duration will both increase substantially to facilitate glycocalyx penetration by the shorter microvilli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.