ArticleMolecular and cellular biochemistry2001
Selective impairment of protein kinase C isotypes in murine macrophage by Leishmania donovani.
Article in Molecular and cellular biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Review on macrophage polarization during visceral leishmaniasis and impact of glycoprotein.Glycoconjugate journal · 2026Review
- Manipulation of macrophage signaling byVirulence · 2025Review
- An Update on Protein Kinases as Therapeutic Targets-Part I: Protein Kinase C Activation and Its Role in Cancer and Cardiovascular Diseases.International journal of molecular sciences · 2023Review
- Characterization of the Protein Tyrosine Phosphatase LmPRL-1 Secreted by Leishmania major via the Exosome Pathway.Infection and immunity · 2017Article
- Function of Macrophage and Parasite Phosphatases in Leishmaniasis.Frontiers in immunology · 2017Review
- The curative effect of fucoidan on visceral leishmaniasis is mediated by activation of MAP kinases through specific protein kinase C isoforms.Cellular & molecular immunology · 2014Article
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- Article
- Mechanisms of immune evasion in leishmaniasis.Advances in applied microbiology · 2013Article
- Immunopathogenesis of non-healing American cutaneous leishmaniasis and progressive visceral leishmaniasis.Seminars in immunopathology · 2012Review
- Endobiont viruses sensed by the human host - beyond conventional antiparasitic therapy.PloS one · 2012Article
- Evasion of Host Defence by Leishmania donovani: Subversion of Signaling Pathways.Molecular biology international · 2011Article
- Leishmania interferes with host cell signaling to devise a survival strategy.Journal of biomedicine & biotechnology · 2010Review
- Differential regulation of protein kinase C isoforms of macrophages by pathogenic and non-pathogenic mycobacteria.Molecular and cellular biochemistry · 2008Article
- Leishmania donovani-induced ceramide as the key mediator of Akt dephosphorylation in murine macrophages: role of protein kinase Czeta and phosphatase.Infection and immunity · 2007Article
- Regulation of impaired protein kinase C signaling by chemokines in murine macrophages during visceral leishmaniasis.Infection and immunity · 2005Article
- Subversion mechanisms by which Leishmania parasites can escape the host immune response: a signaling point of view.Clinical microbiology reviews · 2005Review
- Generation of ceramide in murine macrophages infected with Leishmania donovani alters macrophage signaling events and aids intracellular parasitic survival.Molecular and cellular biochemistry · 2001Article
- Immunomodulatory role of interleukin-10 in visceral leishmaniasis: defective activation of protein kinase C-mediated signal transduction events.Infection and immunity · 2001Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmania donovani, an obligate intracellular parasite resides and multiplies within macrophage of the reticuloendothelial system. The intracellular signalling mechanism involved in the impaired oxidative response in leishmaniasis has not yet been clearly established. Generation of superoxide anion (O2-) is supposed to be the first line of host defence during microbial invasion. We found a substantial inhibition of superoxide anion generation in parasitized macrophages, which was just the reverse in case of macrophages challenged with Lipophosphoglycan (LPG) deficient attenuated leishmanial parasite UR-6. The generation of O2- essentially needs the prior activation of protein kinase C (PKC) mediated phosphorylation events. Our study proposed that phosphorylation of 67, 54, 47 and 36 kDa proteins was attenuated during infection. This was supported by PKC activity study, where Ca-dependent PKC activity was inhibited but, Ca-independent PKC activity was enhanced. This result was further confirmed by using isotype specific pseudosubstrate inhibitors of Ca-dependent PKC beta and Ca-independent PKC zeta. Application of beta-pseudosubstrate could not alter the Ca-dependent PKC activity but zeta-pseudosubstrate inhibited the Ca-independent PKC activity in infected macrophages. Our immunoblot analysis with specific antibody against PKC beta and PKC zeta isotypes showed down regulation of PKC beta-II expression with concomitant induction of PKC zeta. Such inhibition of Ca-dependent PKC beta was reversed in macrophages treated with UR-6. Taken together, our observations revealed that infection with L. donovani selectively attenuates both the expression and activity of Ca-dependent PKC beta.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.