ArticleJournal of clinical pathology2001
Detection of herpesvirus DNA by the polymerase chain reaction (PCR) in vitreous samples from patients with necrotising retinitis.
Article in Journal of clinical pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Defense against HSV-1 in a murine model is mediated by iNOS and orchestrated by the activation of TLR2 and TLR9 in trigeminal ganglia.Journal of neuroinflammation · 2014Article
- Absence of CCR5 increases neutrophil recruitment in severe herpetic encephalitis.BMC neuroscience · 2013Article
- Toll-like receptor (TLR) 2 and TLR9 expressed in trigeminal ganglia are critical to viral control during herpes simplex virus 1 infection.The American journal of pathology · 2010Article
- Lethal encephalitis in myeloid differentiation factor 88-deficient mice infected with herpes simplex virus 1.The American journal of pathology · 2005Article
- [Practical advice for recovery and successful processing of anterior chamber puncture and vitrectomy samples].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2003Article
- Polymerase chain reaction analysis of aqueous humour samples in necrotising retinitis.The British journal of ophthalmology · 2003Article
- Evaluation of microbiota associated with Herpesviruses in active sites of generalized aggressive periodontitis.Annali di stomatologiaArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsViral uveitis and retinitis, usually caused by herpesviruses, are common in immunosuppressed patients. The diagnosis of viral anterior uveitis and retinitis is usually clinical. The polymerase chain reaction (PCR) has been used for the diagnosis of some viral infections, especially those caused by herpesviruses. This paper reports the use of PCR in the diagnosis of viral retinitis in vitreous samples from Brazilian patients.
methodsPCR was used for the diagnosis of necrotising retinitis in vitreous samples from patients from the Hospital São Geraldo, Universidade Federal de Minas Gerais, Brazil. The vitreous samples were collected by paracentesis and stored until analysis. Samples were analysed by PCR using specific primers designed to amplify herpes simplex virus 1 (HSV-1), varicella zoster virus (VZV), or human cytomegalovirus (HCMV). In a case of anterior uveitis, PCR was performed with a sample from the anterior chamber.
resultsHerpesvirus DNA was amplified in 11 of 17 samples. HCVM DNA was detected in nine samples but DNA from HSV-1 and VZV were detected only once each.
conclusionThese results strongly suggest that PCR could be used for a rapid complementary diagnosis of viral uveitis and retinitis. A prospective study to evaluate the PCR results, clinical evolution, and treatment is imperative to corroborate the real value of PCR in diagnosis and how it could help the clinicians' approach.
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