ArticleRNA (New York, N.Y.)2001
A ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formation.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Model systems: how chemical biologists study RNA.Current opinion in chemical biology · 2009Review
- Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.Molecular bioSystems · 2009Review
- Identification and characterization of a short 2'-3' bond-forming ribozyme.RNA (New York, N.Y.) · 2009Article
- A critical assessment of the utility of protein-free splicing systems.RNA (New York, N.Y.) · 2009Article
- An evaluation of detection methods for large lariat RNAs.RNA (New York, N.Y.) · 2005Article
- Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase.Nucleic acids research · 2005Article
- In vitro selection of an archaeal RNase P RNA mimics natural variation.Archaea (Vancouver, B.C.) · 2004Article
- Practical and general synthesis of 5'-adenylated RNA (5'-AppRNA).RNA (New York, N.Y.) · 2004Article
- Inhibition of pre-mRNA splicing by synthetic branched nucleic acids.Nucleic acids research · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
In vitro selection was used to sample SnRNA-related sequences for ribozyme activities, and several 2',5'-branch-forming ribozymes were isolated. One such ribozyme is highly dependent upon an 11-nt motif that contains a conserved U6 snRNA sequence (ACAGAGA-box) known to be important for pre-mRNA splicing. The ribozyme reaction is similar to the first step of splicing in that an internal 2'-hydroxyl of an unpaired adenosine attacks at the 5'-phosphate of a guanosine. It differs in that the leaving group is diphosphate rather than a 5' exon. The finding that lariat formation can be accomplished by a small RNA with sequences related to U6 snRNA indicates that the RNA available in the spliceosome may be involved in RNA-catalyzed branch formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.