ArticleRNA (New York, N.Y.)2001
Replicating satellite RNA induces sequence-specific DNA methylation and truncated transcripts in plants.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 citations in OpenAlex.
- Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants.Nature communications · 2022Article
- Article
- Full-Length Hairpin RNA Accumulates at High Levels in Yeast but Not in Bacteria and Plants.Genes · 2019Article
- Phosphatidylinositol 3-Kinase-DNA Methyltransferase 1-miR-1281-Histone Deacetylase 4 Regulatory Axis Mediates Platelet-Derived Growth Factor-Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells.Journal of the American Heart Association · 2018Article
- Interfering Satellite RNAs ofFrontiers in microbiology · 2017Review
- Expression Analysis of Hairpin RNA CarryingBioMed research international · 2017Article
- Satellite RNAs interfere with the function of viral RNA silencing suppressors.Frontiers in plant science · 2015Article
- Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.PLoS genetics · 2015Article
- C. elegans RNA-dependent RNA polymerases rrf-1 and ego-1 silence Drosophila transgenes by differing mechanisms.Cellular and molecular life sciences : CMLS · 2013Article
- Revisiting RNA-directed DNA methylation.RNA biology · 2013Article
- Inter-species grafting caused extensive and heritable alterations of DNA methylation in Solanaceae plants.PloS one · 2013Article
- Epigenetic mechanisms of genomic imprinting: common themes in the regulation of imprinted regions in mammals, plants, and insects.Genetics research international · 2012Article
- Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation.Journal of virology · 2011Article
- Viral small interfering RNAs target host genes to mediate disease symptoms in plants.PLoS pathogens · 2011Article
- Transgene expression and transgene-induced silencing in diploid and autotetraploid Arabidopsis.Genetics · 2011Article
- A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco.Plant molecular biology · 2010Article
- SPT5-like, a new component in plant RdDM.EMBO reports · 2009Article
- Hairpin RNAs derived from RNA polymerase II and polymerase III promoter-directed transgenes are processed differently in plants.RNA (New York, N.Y.) · 2008Article
- The Caenorhabditis elegans rsd-2 and rsd-6 genes are required for chromosome functions during exposure to unfavorable environments.Genetics · 2008Article
- Conserved themes in small-RNA-mediated transposon control.Trends in cell biology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tobacco plants were transformed with a chimeric transgene comprising sequences encoding beta-glucuronidase (GUS) and the satellite RNA (satRNA) of cereal yellow dwarf luteovirus. When transgenic plants were infected with potato leafroll luteovirus (PLRV), which replicated the transgene-derived satRNA to a high level, the satellite sequence of the GUS:Sat transgene became densely methylated. Within the satellite region, all 86 cytosines in the upper strand and 73 of the 75 cytosines in the lower strand were either partially or fully methylated. In contrast, very low levels of DNA methylation were detected in the satellite sequence of the transgene in uninfected plants and in the flanking nonsatellite sequences in both infected and uninfected plants. Substantial amounts of truncated GUS:Sat RNA accumulated in the satRNA-replicating plants, and most of the molecules terminated at nucleotides within the first 60 bp of the satellite sequence. Whereas this RNA truncation was associated with high levels of satRNA replication, it appeared to be independent of the levels of DNA methylation in the satellite sequence, suggesting that it is not caused by methylation. All the sequenced GUS:Sat DNA molecules were hypermethylated in plants with replicating satRNA despite the phloem restriction of the helper PLRV. Also, small, sense and antisense approximately 22 nt RNAs, derived from the satRNA, were associated with the replicating satellite. These results suggest that the sequence-specific DNA methylation spread into cells in which no satRNA replication occurred and that this was mediated by the spread of unamplified satRNA and/or its associated 22 nt RNA molecules.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.