ArticleRNA (New York, N.Y.)2001
Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- Article
- A novel role of U1 snRNP: Splice site selection from a distance.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- Article
- Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull.RNA (New York, N.Y.) · 2015Article
- RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation.The EMBO journal · 2011Article
- G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site.The Journal of biological chemistry · 2011Article
- DExD/H-box Prp5 protein is in the spliceosome during most of the splicing cycle.RNA (New York, N.Y.) · 2009Article
- A targeted bypass screen identifies Ynl187p, Prp42p, Snu71p, and Cbp80p for stable U1 snRNP/Pre-mRNA interaction.Molecular and cellular biology · 2009Article
- Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA.Nature structural & molecular biology · 2009Article
- The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing.Nucleic acids research · 2008Article
- Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.The Journal of cell biology · 2007Article
- Cryptic splice site usage in exon 7 of the human fibrinogen Bbeta-chain gene is regulated by a naturally silent SF2/ASF binding site within this exon.RNA (New York, N.Y.) · 2006Article
- Determinants of the inherent strength of human 5' splice sites.RNA (New York, N.Y.) · 2005Article
- Effects of the U1C L13 mutation and temperature regulation of yeast commitment complex formation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- New tertiary constraints between the RNA components of active yeast spliceosomes: a photo-crosslinking study.RNA (New York, N.Y.) · 2004Article
- Comparative analysis detects dependencies among the 5' splice-site positions.RNA (New York, N.Y.) · 2004Article
- hnRNP H binding at the 5' splice site correlates with the pathological effect of two intronic mutations in the NF-1 and TSHbeta genes.Nucleic acids research · 2004Article
- A novel approach to describe a U1 snRNA binding site.Nucleic acids research · 2003Article
- Intrinsic differences between authentic and cryptic 5' splice sites.Nucleic acids research · 2003Article
- U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5' splice sites.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Base pairing between the 5' end of U1 snRNA and the conserved 5' splice site of pre-mRNA is important for commitment complex formation in vitro. However, the biochemical mechanisms by which pre-mRNA is initially recognized by the splicing machinery is not well understood. To evaluate the role of this base pairing interaction, we truncated U1 snRNA to eliminate the RNA-RNA interaction and surprisingly found that U1 snRNP can still form a nearly normal RNA-protein complex and maintain sequence specificity. We propose that some feature of U1 snRNP, perhaps one or more protein factors, is more important than the base pairing for initial 5' splice site recognition. In addition, at least five sets of interactions contribute to complex formation or stability. Only one of these is base pairing between the 5' splice site and the 5' end of U1 snRNA, without which the U1 snRNP-pre-mRNA complex is less stable and has a somewhat altered conformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.