ArticleIn vitro cellular & developmental biology. Animal2000
Mammary fibroblasts stimulate growth, alveolar morphogenesis, and functional differentiation of normal rat mammary epithelial cells.
Article in In vitro cellular & developmental biology. Animal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats.International journal of molecular sciences · 2023Article
- Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks.Journal of mammary gland biology and neoplasia · 2020Review
- Stromal fibroblasts derived from mammary gland of bovine with mastitis display inflammation-specific changes.Scientific reports · 2016Article
- A co-culture genome-wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation.Breast cancer research : BCR · 2015Article
- Hormone-responsive 3D multicellular culture model of human breast tissue.Biomaterials · 2012Article
- Adipocyte derived paracrine mediators of mammary ductal morphogenesis controlled by retinoic acid receptors.Developmental biology · 2011Article
- Synthetic adipose tissue models for studying mammary gland development and breast tissue engineering.Journal of mammary gland biology and neoplasia · 2010Review
- Morphogenesis of the developing mammary gland: stage-dependent impact of adipocytes.Developmental biology · 2010Article
- A complex 3D human tissue culture system based on mammary stromal cells and silk scaffolds for modeling breast morphogenesis and function.Biomaterials · 2010Article
- Preadipocytes stimulate ductal morphogenesis and functional differentiation of human mammary epithelial cells on 3D silk scaffolds.Tissue engineering. Part A · 2009Article
- Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors.Breast cancer research : BCR · 2007Article
- Stromal-epithelial interactions in aging and cancer: senescent fibroblasts alter epithelial cell differentiation.Journal of cell science · 2005Article
- Use of three-dimensional basement membrane cultures to model oncogene-induced changes in mammary epithelial morphogenesis.Journal of mammary gland biology and neoplasia · 2004Review
- Development of the mammary gland requires DGAT1 expression in stromal and epithelial tissues.Development (Cambridge, England) · 2004Article
- Differentiation of prostate epithelial cell cultures by matrigel/ stromal cell glandular reconstruction.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Stromal-epithelial interactions play a profound role in regulating normal and tumor development in the mammary gland. The molecular details of these events, however, are incompletely understood. A novel serum-free transwell coculture system was developed to study the natural paracrine interactions between mammary epithelial cells (MEC) and mammary fibroblasts (MFC) isolated from normal rats during puberty. The MEC were cultured within a reconstituted basement membrane (RBM) in transwell inserts with or without MFC in the lower well. The presence of MFC stimulated epithelial cell growth, induced alveolar morphogenesis, and enhanced casein accumulation, a marker of the functional differentiation of MEC, but did not induce ductal morphogenesis. Potent mitogenic, morphogenic, and lactogenic effects were observed when the MFC were cultured either on plastic or within a layer of RBM. Although most MFC maintained on plastic died after 1 wk in serum-free medium, fibroblast survival was enhanced significantly when the MFC were cultured within the RBM. Taken together, this in vitro model effectively reconstitutes a physiologically relevant three-dimensional microenvironment for MEC and MFC, and seems ideal for studying the locally derived factors that regulate the developmental fate of the epithelial and fibroblast compartments of the mammary gland.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.