ArticleProceedings of the National Academy of Sciences of the United States of America2001
CpG island protects Rous sarcoma virus-derived vectors integrated into nonpermissive cells from DNA methylation and transcriptional suppression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Article
- Remembering Jan Svoboda: A Personal Reflection.Viruses · 2018Article
- Accumulation of long-term transcriptionally active integrated retroviral vectors in active promoters and enhancers.Nucleic acids research · 2017Article
- Regulation of DNA demethylation by the XPC DNA repair complex in somatic and pluripotent stem cells.Genes & development · 2017Article
- A minimal ubiquitous chromatin opening element (UCOE) effectively prevents silencing of juxtaposed heterologous promoters by epigenetic remodeling in multipotent and pluripotent stem cells.Nucleic acids research · 2015Article
- Embryo development, fetal growth and postnatal phenotype of eGFP lambs generated by lentiviral transgenesis.Transgenic research · 2015Article
- Alpharetroviral vectors: from a cancer-causing agent to a useful tool for human gene therapy.Viruses · 2014Review
- Retroviral DNA methylation and epigenetic repression are mediated by the antiviral host protein Daxx.Journal of virology · 2013Article
- Epigenetic interplay between mouse endogenous retroviruses and host genes.Genome biology · 2012Article
- Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site.Nucleic acids research · 2012Article
- Paucity of HIV DNA methylation in latently infected, resting CD4+ T cells from infected individuals receiving antiretroviral therapy.Journal of virology · 2012Article
- Adeno-associated viral vector-mediated transgene expression is independent of DNA methylation in primate liver and skeletal muscle.PloS one · 2011Article
- In vivo, in vitro, and in silico analysis of methylation of the HIV-1 provirus.Methods (San Diego, Calif.) · 2011Review
- Cell line-dependent variability in HIV activation employing DNMT inhibitors.Virology journal · 2010Article
- Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas.Journal of virology · 2010Article
- CpG methylation controls reactivation of HIV from latency.PLoS pathogens · 2009Article
- The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing.Journal of virology · 2008Article
- High-frequency epigenetic repression and silencing of retroviruses can be antagonized by histone deacetylase inhibitors and transcriptional activators, but uniform reactivation in cell clones is restricted by additional mechanisms.Journal of virology · 2007Article
- Plasmid engineering for controlled and sustained gene expression for nonviral gene therapy.Pharmaceutical research · 2006Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CpG islands are important in the protection of adjacent housekeeping genes from de novo DNA methylation and for keeping them in a transcriptionally active state. However, little is known about their capacity to protect heterologous genes and assure position-independent transcription of adjacent transgenes or retroviral vectors. To tackle this question, we have used the mouse aprt CpG island to flank a Rous sarcoma virus (RSV)-derived reporter vector and followed the transcriptional activity of integrated vectors. RSV is an avian retrovirus which does not replicate in mammalian cells because of several blocks at all levels of the replication cycle. Here we show that our RSV-derived reporter proviruses linked to the mouse aprt gene CpG island remain undermethylated and keep their transcriptional activity after stable transfection into both avian and nonpermissive mammalian cells. This effect is most likely caused by the protection from de novo methylation provided by the CpG island and not by enhancement of the promoter strength. Our results are consistent with previous finding of CpG islands in proximity to active but not inactive proviruses and support further investigation of the protection of the gene transfer vectors from DNA methylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.