ArticleThe Journal of physiology2001
Origin and propagation of spontaneous excitation in smooth muscle of the guinea-pig urinary bladder.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 128 citations in OpenAlex.
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- Modulation of intracellular calcium activity in interstitial cells of Cajal by inhibitory neural pathways within the internal anal sphincter.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Spontaneous synaptic drive in detrusor smooth muscle: computational investigation and implications for urinary bladder function.Journal of computational neuroscience · 2019Article
- The KThe Journal of physiology · 2019Article
- Muscle cells, nerves, fibroblasts and vessels in the detrusor of the rat urinary bladder.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2019Article
- Modulation of Bladder Wall Micromotions Alters Intravesical Pressure Activity in the Isolated Bladder.Frontiers in physiology · 2018Article
- Investigation of the Syncytial Nature of Detrusor Smooth Muscle as a Determinant of Action Potential Shape.Frontiers in physiology · 2018Article
- Role of prostatic interstitial cells in prostate motility.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2017Review
- Structural and functional changes in gap junctional intercellular communication in a rat model of overactive bladder syndrome induced by partial bladder outlet obstruction.Experimental and therapeutic medicine · 2016Article
- BK channel regulation by phosphodiesterase type 1: a novel signaling pathway controlling human detrusor smooth muscle function.American journal of physiology. Renal physiology · 2016Article
- The actions of prolonged exposure to cholinergic agonists on isolated bladder strips from the rat.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Functional link between muscarinic receptors and large-conductance Ca2+ -activated K+ channels in freshly isolated human detrusor smooth muscle cells.Pflugers Archiv : European journal of physiology · 2015Article
- A computational model of urinary bladder smooth muscle syncytium : validation and investigation of electrical properties.Journal of computational neuroscience · 2015Article
- L-type Ca2+ channel sparklets revealed by TIRF microscopy in mouse urinary bladder smooth muscle.PloS one · 2014Article
- KV2.1 and electrically silent KV channel subunits control excitability and contractility of guinea pig detrusor smooth muscle.American journal of physiology. Cell physiology · 2012Article
- Cellular expression profile for interstitial cells of cajal in bladder - a cell often misidentified as myocyte or myofibroblast.PloS one · 2012Article
- The validation of a functional, isolated pig bladder model for physiological experimentation.Frontiers in pharmacology · 2012Article
- Sex- and age-related changes in connexin 43 expression in normal rat bladder.International neurourology journal · 2011Article
- Aging differentially modifies agonist-evoked mouse detrusor contraction and calcium signals.Age (Dordrecht, Netherlands) · 2011Article
- Role of KIT-Positive Interstitial Cells of Cajal in the Urinary Bladder and Possible Therapeutic Target for Overactive Bladder.Advances in urology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The origin and propagation of waves of spontaneous excitation in bundles of smooth muscle of the guinea-pig bladder were examined using intracellular recording techniques and visualization of the changes in the intracellular calcium concentration ([Ca2+]i). Bladder smooth muscle cells exhibited spontaneous transient increases in [Ca2+]i which originated along a boundary of each smooth muscle bundle and then spread to the other boundary with a conduction velocity of 2.0 1r1r>mm1> s-1. Spontaneous increases in [Ca2+]i were always preceded by action potentials. Nifedipine (10 microM) abolished increases in both [Ca2+]i and action potentials. Caffeine (10 1s1sFmM1F), ryanodine (50 microM) and cyclopiazonic acid (10 microM reduced the amplitude of the associated increases in [Ca2+]i without preventing the generation of action potentials. Spontaneous action potentials had conduction velocities of 40 1t1t>mm 1> s-1 in the axial direction and 1.3 1u1u>mm 1> s-1 in the transverse direction. The electrical length constants of the bundles of muscle were 425 microM in the axial direction and 12.5 microM in the transverse direction. Neurobiotin, injected into an impaled smooth muscle cell, spread more readily to neighbouring cells located in the axial direction than those located in the transverse direction. The spread of neurobiotin was inhibited by 18beta-glycyrrhetinic acid (18beta-GA, 40 microM), a gap junction blocker. Immunohistochemistry for Connexin 43 showed abundant punctate staining on the smooth muscle cell membranes. These results suggested that spontaneous action potentials and associated calcium waves occur almost simultaneously along the boundary of bladder smooth muscle bundles and then propagate to the other boundary probably through gap junctions.
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