ArticleJournal of bacteriology2001
Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Characterization of Treponema denticola mutants defective in the major antigenic proteins, Msp and TmpC.PloS one · 2014Article
- Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection.International journal of dentistry · 2012Article
- Deciphering morphological determinants of the helix-shaped Leptospira.Journal of bacteriology · 2011Article
- Virulence factors of the oral spirochete Treponema denticola.Journal of dental research · 2011Review
- Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography.Journal of molecular biology · 2010Article
- Electron cryotomography.Cold Spring Harbor perspectives in biology · 2010Review
- Killing of Treponema denticola by mouse peritoneal macrophages.Journal of dental research · 2010Article
- Cryo-electron tomography elucidates the molecular architecture of Treponema pallidum, the syphilis spirochete.Journal of bacteriology · 2009Article
- Electron cryotomography: a new view into microbial ultrastructure.Current opinion in microbiology · 2009Review
- Native cellular architecture of Treponema denticola revealed by cryo-electron tomography.Journal of structural biology · 2008Article
- Treponema denticola in disseminating endodontic infections.Journal of dental research · 2006Article
- Borrelia burgdorferi ftsZ plays a role in cell division.Journal of bacteriology · 2006Article
- The scc spirochetal coiled-coil protein forms helix-like filaments and binds to nucleic acids generating nucleoprotein structures.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Treponema denticola, a ribbon-like structure of cytoplasmic filaments spans the cytoplasm at all stages of the cell division process. Insertional inactivation was used as a first step to determine the function of the cytoplasmic filaments. A suicide plasmid was constructed that contained part of cfpA and a nonpolar erythromycin resistance cassette (ermF and ermAM) inserted near the beginning of the gene. The plasmid was electroporated into T. denticola, and double-crossover recombinants which had the chromosomal copy of cfpA insertionally inactivated were selected. Immunoblotting and electron microscopy confirmed the lack of cytoplasmic filaments. The mutant was further analyzed by dark-field microscopy to determine cell morphology and by the binding of two fluorescent dyes to DNA to assess the distribution of cellular nucleic acids. The cytoplasmic filament protein-deficient mutant exhibited pleiotropic defects, including highly condensed chromosomal DNA, compared to the homogeneous distribution of the DNA throughout the cytoplasm in a wild-type cell. Moreover, chains of cells are formed by the cytoplasmic filament-deficient mutant, and those cells show reduced spreading in agarose, which may be due to the abnormal cell length. The chains of cells and the highly condensed chromosomal DNA suggest that the cytoplasmic filaments may be involved in chromosome structure, segregation, or the cell division process in Treponema.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.