ArticleJournal of bacteriology2001
Periplasmic transit and disulfide bond formation of the autotransported Shigella protein IcsA.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 95 citations in OpenAlex.
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- VirB, a key transcriptional regulator ofbioRxiv : the preprint server for biology · 2023Article
- Cysteine-Dependent Conformational Heterogeneity of Shigella flexneri Autotransporter IcsA and Implications of Its Function.Microbiology spectrum · 2022Article
- VirB, a key transcriptional regulator of virulence plasmid genes inJournal of bacteriology · 2021Article
- Folding Control in the Path of Type 5 Secretion.Toxins · 2021Review
- Two pKM101-encoded proteins, the pilus-tip protein TraC and Pep, assemble on the Escherichia coli cell surface as adhesins required for efficient conjugative DNA transfer.Molecular microbiology · 2019Article
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- Bacterial thiol oxidoreductases - from basic research to new antibacterial strategies.Applied microbiology and biotechnology · 2017Review
- Of linkers and autochaperones: an unambiguous nomenclature to identify common and uncommon themes for autotransporter secretion.Molecular microbiology · 2015Review
- Single-cell characterization of autotransporter-mediated Escherichia coli surface display of disulfide bond-containing proteins.The Journal of biological chemistry · 2012Article
- IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface.Microbial cell factories · 2012Article
- A bioinformatic strategy for the detection, classification and analysis of bacterial autotransporters.PloS one · 2012Article
- Size and conformation limits to secretion of disulfide-bonded loops in autotransporter proteins.The Journal of biological chemistry · 2011Article
- TrbB from conjugative plasmid F is a structurally distinct disulfide isomerase that requires DsbD for redox state maintenance.Journal of bacteriology · 2011Article
- A conserved aromatic residue in the autochaperone domain of the autotransporter Hbp is critical for initiation of outer membrane translocation.The Journal of biological chemistry · 2010Article
- Comparative analysis of the biochemical and functional properties of C-terminal domains of autotransporters.Journal of bacteriology · 2010Article
- Characterization of the O-antigen polymerase (Wzy) of Francisella tularensis.The Journal of biological chemistry · 2010Article
- Article
- Importance of conserved residues of the serine protease autotransporter beta-domain in passenger domain processing and beta-barrel assembly.Infection and immunity · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Shigella outer membrane protein IcsA belongs to the family of type V secreted (autotransported) virulence factors. Members of this family mediate their own translocation across the bacterial outer membrane: the carboxy-terminal beta domain forms a beta barrel channel in the outer membrane through which the amino-terminal alpha domain passes. IcsA, which is localized at one pole of the bacterium, mediates actin assembly by Shigella, which is essential for bacterial intracellular movement and intercellular dissemination. Here, we characterize the transit of IcsA across the periplasm during its secretion. We show that an insertion in the dsbB gene, whose gene product mediates disulfide bond formation of many periplasmic intermediates, does not affect the surface expression or unipolar targeting of IcsA. However, IcsA forms one disulfide bond in the periplasm in a DsbA/DsbB-dependent fashion. Furthermore, cellular fractionation studies reveal that IcsA has a transient soluble periplasmic intermediate. Our data also suggest that IcsA is folded in a proteinase K-resistant state in the periplasm. From these data, we propose a novel model for the secretion of IcsA that may be applicable to other autotransported proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.