ArticleJournal of bacteriology2001
Diel expression of cell cycle-related genes in synchronized cultures of Prochlorococcus sp. strain PCC 9511.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- In Situ Bacterioplankton Growth Partitioning by High-Resolution Metatranscriptomics.Environmental microbiology reports · 2026Article
- Validation of reference genes for the normalization of RT-qPCR gene expression inIranian journal of microbiology · 2022Article
- Cell Cycle, Filament Growth and Synchronized Cell Division in Multicellular Cable Bacteria.Frontiers in microbiology · 2021Article
- Cell cycle-dependent regulation of FtsZ in Escherichia coli in slow growth conditions.Molecular microbiology · 2018Article
- Article
- Torn apart and reunited: impact of a heterotroph on the transcriptome of Prochlorococcus.The ISME journal · 2016Article
- Transcriptome dynamics of a broad host-range cyanophage and its hosts.The ISME journal · 2016Article
- Diversification of DnaA dependency for DNA replication in cyanobacterial evolution.The ISME journal · 2016Article
- RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress.Scientific reports · 2015Article
- Prochlorococcus: the structure and function of collective diversity.Nature reviews. Microbiology · 2015Review
- Proteome-wide analysis and diel proteomic profiling of the cyanobacterium Arthrospira platensis PCC 8005.PloS one · 2014Article
- Trichodesmium--a widespread marine cyanobacterium with unusual nitrogen fixation properties.FEMS microbiology reviews · 2013Review
- Transcriptome and proteome dynamics of a light-dark synchronized bacterial cell cycle.PloS one · 2012Article
- Prochlorococcus and Synechococcus have Evolved Different Adaptive Mechanisms to Cope with Light and UV Stress.Frontiers in microbiology · 2012Article
- The ftsZ Gene of Mycobacterium smegmatis is expressed Through Multiple Transcripts.The open microbiology journal · 2011Article
- Ultraviolet stress delays chromosome replication in light/dark synchronized cells of the marine cyanobacterium Prochlorococcus marinus PCC9511.BMC microbiology · 2010Article
- Choreography of the transcriptome, photophysiology, and cell cycle of a minimal photoautotroph, prochlorococcus.PloS one · 2009Article
- Fluorescence staining of live cyanobacterial cells suggest non-stringent chromosome segregation and absence of a connection between cytoplasmic and thylakoid membranes.BMC cell biology · 2007Article
- Molecular evolution of ldpA, a gene mediating the circadian input signal in cyanobacteria.Journal of molecular evolution · 2005Article
- Concentration and assembly of the division ring proteins FtsZ, FtsA, and ZipA during the Escherichia coli cell cycle.Journal of bacteriology · 2003Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cell cycle of the chlorophyll b-possessing marine cyanobacterium Prochlorococcus is highly synchronized under natural conditions. To understand the underlying molecular mechanisms we cloned and sequenced dnaA and ftsZ, two key cell cycle-associated genes, and studied their expression. An axenic culture of Prochlorococcus sp. strain PCC 9511 was grown in a turbidostat with a 12 h-12 h light-dark cycle for 2 weeks. During the light periods, a dynamic light regimen was used in order to simulate the natural conditions found in the upper layers of the world's oceans. This treatment resulted in strong cell cycle synchronization that was monitored by flow cytometry. The steady-state mRNA levels of dnaA and ftsZ were monitored at 4-h intervals during four consecutive division cycles. Both genes exhibited clear diel expression patterns with mRNA maxima during the replication (S) phase. Western blot experiments indicated that the peak of FtsZ concentration occurred at night, i.e., at the time of cell division. Thus, the transcript accumulation of genes involved in replication and division is coordinated in Prochlorococcus sp. strain PCC 9511 and might be crucial for determining the timing of DNA replication and cell division.
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