ArticleJournal of bacteriology2001
Ammonia switch-off of nitrogen fixation in the methanogenic archaeon Methanococcus maripaludis: mechanistic features and requirement for the novel GlnB homologues, NifI(1) and NifI(2).
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 89 citations in OpenAlex.
- Article
- Function, Structure, and Regulation of Nitrogen Fixation-like Metalloproteins for Nitrogen, Energy, Carbon, and Sulfur Metabolism.Chemical reviews · 2026Review
- In Vivo Enzyme Catalytic Rates in Formate-Growing Methanococcus maripaludis.Microbial biotechnology · 2026Article
- Nitrogen Fixation and Anoxygenic Photosynthesis in Filamentous Non-Heterocystous Cyanobacterium of the GenusPlants (Basel, Switzerland) · 2025Article
- Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under NScientific reports · 2025Article
- Nitrogen fixation by methanogenic Archaea, literature review and DNA database-based analysis; significance in face of climate change.Archives of microbiology · 2024Review
- Discovery of Eremiobacterota with nifH homologues in tundra soil.Environmental microbiology reports · 2024Article
- Expression of V-nitrogenase and Fe-nitrogenase inApplied and environmental microbiology · 2023Article
- Article
- Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering inACS synthetic biology · 2022Article
- The Oxoglutarate Binding Site and Regulatory Mechanism Are Conserved in Ammonium Transporter Inhibitors GlnKs fromInternational journal of molecular sciences · 2021Article
- Transcriptional response to prolonged perchlorate exposure in the methanogen Methanosarcina barkeri and implications for Martian habitability.Scientific reports · 2021Article
- Illumina sequencing of 16S rRNA genes reveals a unique microbial community in three anaerobic sludge digesters of Dubai.PloS one · 2021Article
- Diazotroph Genomes and Their Seasonal Dynamics in a Stratified Humic Bog Lake.Frontiers in microbiology · 2020Article
- First Observation of an Acetate Switch in a Methanogenic Autotroph (Microbiology insights · 2020Article
- Denitrification Aligns with NScientific reports · 2019Article
- Metabolic processes of Methanococcus maripaludis and potential applications.Microbial cell factories · 2016Review
- Photosynthetic Versatility in the Genome ofFrontiers in microbiology · 2016Article
- Flux measurements and maintenance energy for carbon dioxide utilization by Methanococcus maripaludis.Microbial cell factories · 2015Article
- Evolution of molybdenum nitrogenase during the transition from anaerobic to aerobic metabolism.Journal of bacteriology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Ammonia switch-off is the immediate inactivation of nitrogen fixation that occurs when a superior nitrogen source is encountered. In certain bacteria switch-off occurs by reversible covalent ADP-ribosylation of the dinitrogenase reductase protein, NifH. Ammonia switch-off occurs in diazotrophic species of the methanogenic Archaea as well. We showed previously that in Methanococcus maripaludis switch-off requires at least one of two novel homologues of glnB, a family of genes whose products play a central role in nitrogen sensing and regulation in bacteria. The novel glnB homologues have recently been named nifI(1) and nifI(2). Here we use in-frame deletions and genetic complementation analysis in M. maripaludis to show that the nifI(1) and nifI(2) genes are both required for switch-off. We could not detect ADP-ribosylation or any other covalent modification of dinitrogenase reductase during switch-off, suggesting that the mechanism differs from the well-studied bacterial system. Furthermore, switch-off did not affect nif gene transcription, nifH mRNA stability, or NifH protein stability. Nitrogenase activity resumed within a short time after ammonia was removed from a switched-off culture, suggesting that whatever the mechanism, it is reversible. We demonstrate the physiological importance of switch-off by showing that it allows growth to accelerate substantially when a diazotrophic culture is switched to ammonia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.