ArticleTraffic (Copenhagen, Denmark)2000
Purification of the vertebrate nuclear pore complex by biochemical criteria.
Article in Traffic (Copenhagen, Denmark), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics.eLife · 2025Article
- Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly.The EMBO journal · 2023Article
- Article
- Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites.Nucleus (Austin, Tex.) · 2017Article
- Molecular Characterization and Functional Analysis of Annulate Lamellae Pore Complexes in Nuclear Transport in Mammalian Cells.PloS one · 2015Article
- Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin.Nucleus (Austin, Tex.) · 2015Article
- Nuclear matrix proteome analysis of Drosophila melanogaster.Molecular & cellular proteomics : MCP · 2010Article
- The nuclear envelope.Cold Spring Harbor perspectives in biology · 2010Review
- Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.Molecular biology of the cell · 2009Article
- Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.Molecular biology of the cell · 2008Article
- Nuclear pore disassembly from endoplasmic reticulum membranes promotes Ca2+ signalling competency.The Journal of physiology · 2008Article
- Xenopus importin beta validates human importin beta as a cell cycle negative regulator.BMC cell biology · 2008Article
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- Cdk1 and okadaic acid-sensitive phosphatases control assembly of nuclear pore complexes in Drosophila embryos.Molecular biology of the cell · 2005Article
- Autoantigens of the nuclear pore complex.Journal of molecular medicine (Berlin, Germany) · 2004Review
- Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.Molecular biology of the cell · 2003Article
- Isolation and characterization of new Saccharomyces cerevisiae mutants perturbed in nuclear pore complex assembly.BMC genetics · 2002Article
- Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation.The Journal of cell biology · 2002Article
- Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.The Journal of cell biology · 2001Article
- The nuclear pore complex.Genome biology · 2001Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The nuclear pore is a large and complex biological machine, mediating all signal-directed transport between the nucleus and the cytoplasm. The vertebrate pore has a mass of approximately 120 million daltons or 30 times the size of a ribosome. The large size of the pore, coupled to its tight integration in the nuclear lamina, has hampered the isolation of pore complexes from vertebrate sources. We have now developed a strategy for the purification of nuclear pores from in vitro assembled annulate lamellae (AL), a cytoplasmic mimic of the nuclear envelope that lacks a lamina, nuclear matrix, and chromatin-associated proteins. We find that purified pore complexes from annulate lamellae contain every nuclear pore protein tested. In addition, immunoblotting reveals the presence of soluble transport receptors and factors known to play important roles in the transport of macromolecules through the pore. While transport factors such as Ran and NTF2 show only transient interaction with the pores, a number of soluble transport receptors, including importin beta, show a tight association with the purified pores. In summary, we report that we have purified the vertebrate pore by biochemical criteria; silver staining reveals approximately 40-50 distinct protein bands.
Indexed as
Identifiers
11208084PMC4456041W2098949217What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.