ArticleBritish journal of cancer2001
Human bladder cancer invasion model using rat bladder in vitro and its use to test mechanisms and therapeutic inhibitors of invasion.
Article in British journal of cancer, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Phase I trial of intravesical Suramin in recurrent superficial transitional cell bladder carcinoma.British journal of cancer · 2005Trial
- CRISPR and compound screens in a novel ex vivo tissue model identify DDR1 and ETA as regulators of cancer cell invasion.Cellular & molecular biology letters · 2026Article
- GALNT6 promotes bladder cancer malignancy and immune escape by epithelial-mesenchymal transition and CD8Cancer cell international · 2024Article
- Sulforaphane and bladder cancer: a potential novel antitumor compound.Frontiers in pharmacology · 2023Review
- Intratumoral Heterogeneity Promotes Collective Cancer Invasion through NOTCH1 Variation.Cells · 2021Article
- Chemoresection by mitomycin C compared to transurethral resection of bladder tumor in patients with recurrent nonmuscle-invasive bladder cancer: A systematic review and meta-analysis.Turkish journal of urology · 2021Article
- Grainyhead-Like 3 Influences Migration and Invasion of Urothelial Carcinoma Cells.International journal of molecular sciences · 2021Article
- The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Changes in autofluorescence based organoid model of muscle invasive urinary bladder cancer.Biomedical optics express · 2016Article
- RGD-modifided oncolytic adenovirus exhibited potent cytotoxic effect on CAR-negative bladder cancer-initiating cells.Cell death & disease · 2015Article
- Expression of Robo protein in bladder cancer tissues and its effect on the growth of cancer cells by blocking Robo protein.International journal of clinical and experimental pathology · 2015Article
- Current animal models of bladder cancer: Awareness of translatability (Review).Experimental and therapeutic medicine · 2014Article
- Expression of the E-cadherin repressors Snail, Slug and Zeb1 in urothelial carcinoma of the urinary bladder: relation to stromal fibroblast activation and invasive behaviour of carcinoma cells.Histochemistry and cell biology · 2012Article
- Emerging intravesical therapies for management of nonmuscle invasive bladder cancer.Open access journal of urology · 2010Review
- DBC1 re-expression alters the expression of multiple components of the plasminogen pathway.Oncogene · 2006Article
- Cooperative stimulation of vascular endothelial growth factor expression by hypoxia and reactive oxygen species: the effect of targeting vascular endothelial growth factor and oxidative stress in an orthotopic xenograft model of bladder carcinoma.British journal of cancer · 2005Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As well as being a passive support, the extracellular matrix also regulates key biological processes such as invasion, differentiation and angiogenesis. We have therefore developed an in vitro model of bladder cancer invasion using de-epithelialized rat bladder to allow for tumour cell-extracellular matrix interactions. Onto this we have seeded a panel of human bladder cancer cell lines (RT4, RT112, 253J and EJ28 (T24)) representing progression from well to poorly differentiated phenotypes and used as models of superficial to invasive bladder cancer. The better differentiated cell lines RT4 and RT112 reproducibly grew as stratified epithelium, whereas poorly differentiated EJ28 cells invaded across a broad front. Invasion was not simply related to proliferation rate, measured either as doubling time on plastic (non-invasive 253J and invasive EJ28 having the same doubling time) or by Ki-67 proliferation index within the model. We used the model to test the ability of 4 compounds that interfere with tumour cell-extracellular matrix interactions (suramin, N-acetylcysteine and the urokinase plasminogen activator pathway antagonists A5 compound and monoclonal antibody Mab 3936) to inhibit invasion. At non-toxic concentrations, all significantly inhibited invasion (P< 0.05), although to varying degrees, suramin and A5 almost completely and N-acetylcysteine the least. In conclusion, this model shows the urokinase system is important for bladder invasion and can be used to investigate other mechanisms of bladder cancer invasion and also for the testing of intravesical drugs.
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