ArticleTransgenic research2000
Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (Oreochromis niloticus).
Article in Transgenic research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Cloning, characterization, and transcriptional activity of β-actin promoter of African catfish (Clarias gariepinus).Molecular biology reports · 2021Article
- Sexually Dimorphic Growth Stimulation in a Strain of Growth Hormone Transgenic Coho Salmon (Oncorhynchus kisutch).Marine biotechnology (New York, N.Y.) · 2021Article
- Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells.Transgenic research · 2014Article
- Relative transgene expression frequencies in homozygous versus hemizygous transgenic mice.Transgenic research · 2013Article
- Characterization of estrogen-responsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogenous choriogenin H promoter.Transgenic research · 2013Article
- Tol2-mediated generation of a transgenic haplochromine cichlid, Astatotilapia burtoni.PloS one · 2013Article
- Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena.Transgenic research · 2011Article
- Tol2-mediated transgenesis in tilapia (Oreochromis niloticus).Aquaculture (Amsterdam, Netherlands) · 2011Article
- The growth hormone-encoding gene isolated and characterized from Labeo rohita Hamilton is expressed in CHO cells under the control of constitutive promoters in 'autotransgene' constructs.Fish physiology and biochemistry · 2008Article
- Transgene constructs in coho salmon (Oncorhynchus kisutch) are repeated in a head-to-tail fashion and can be integrated adjacent to horizontally-transmitted parasite DNA.Transgenic research · 2006Article
- Analysis of cell-specificity and variegation of transgene expression driven by salmon prolactin promoter in stable lines of transgenic rainbow trout.Transgenic research · 2003Article
- Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis).Journal of biosciences · 2001Article
- Site-directed gene integration in transgenic zebrafish mediated by cre recombinase using a combination of mutant lox sites.Marine biotechnology (New York, N.Y.)Article
- Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be related to transgene copy number. Mosaic patterns of somatic lacZ expression were observed in these three lines which differed between lines but were consistent within a line. We also observed that expression of the reporter gene in homozygous transgenic fish was approximately two-fold greater than in the hemizygous transgenics. Analysis of expression of the reporter gene on a tissue-to-tissue basis demonstrated that lacZ expression of the reporter gene in stably transformed fish occured with variable intensity in different organs and tissues and was also sometimes variable in different cells of the same tissue in Gland G2 generations of the transgenic lines.
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