ArticlePlant molecular biology2000
Molecular cloning and functional expression of a stress-induced multifunctional O-methyltransferase with pinosylvin methyltransferase activity from Scots pine (Pinus sylvestris L.).
Article in Plant molecular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 82 citations in OpenAlex.
- PmWRKY-10 Enhances Pine Wilt Disease Resistance by Promoting Pinosylvin Monomethyl Ether Biosynthesis Through Transcriptional Activation of PmPMT2-1.Molecular plant pathology · 2026Article
- AFrontiers in plant science · 2024Article
- Production of Nematicidal Pinosylvin Stilbenes in Cell Suspension Cultures ofPlants (Basel, Switzerland) · 2022Article
- Enhanced production of pinosylvin stilbene with aging of Pinus strobus callus and nematicidal activity of callus extracts against pinewood nematodes.Scientific reports · 2022Article
- Rational Design of Resveratrol O-methyltransferase for the Production of Pinostilbene.International journal of molecular sciences · 2021Article
- Impact of Environmental Factors on Stilbene Biosynthesis.Plants (Basel, Switzerland) · 2021Review
- Article
- Regulation of stilbene biosynthesis in plants.Planta · 2017Review
- Developmental Changes in Scots Pine Transcriptome during Heartwood Formation.Plant physiology · 2016Article
- De novo sequencing and analysis of Lophophora williamsii transcriptome, and searching for putative genes involved in mescaline biosynthesis.BMC genomics · 2015Article
- Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli.BMC biotechnology · 2014Article
- A farnesoic acid O-methyltransferase (FAMeT) from Exopalaemon carinicauda is responsive to Vibrio anguillarum and WSSV challenge.Cell stress & chaperones · 2014Article
- The R2R3-MYB transcription factors MYB14 and MYB15 regulate stilbene biosynthesis in Vitis vinifera.The Plant cell · 2013Article
- De novo characterization of Larix gmelinii (Rupr.) Rupr. transcriptome and analysis of its gene expression induced by jasmonates.BMC genomics · 2013Article
- Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei.BMC evolutionary biology · 2013Article
- Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene.Protoplasma · 2012Article
- Characterization of three O-methyltransferases involved in noscapine biosynthesis in opium poppy.Plant physiology · 2012Article
- Defense mechanisms against herbivory in Picea: sequence evolution and expression regulation of gene family members in the phenylpropanoid pathway.BMC genomics · 2011Article
- A stress-inducible resveratrol O-methyltransferase involved in the biosynthesis of pterostilbene in grapevine.Plant physiology · 2008Article
- EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol.The Plant cell · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formation of pinosylvin (PS) and pinosylvin 3-O-monomethyl ether (PSM), as well as the activities of stilbene synthase (STS) and S-adenosyl-1-methionine (SAM):pinosylvin O-methyltransferase (PMT), were induced strongly in needles of Scots pine seedlings upon ozone treatment, as well as in cell suspension cultures of Scots pine upon fungal elicitation. A SAM-dependent PMT protein was purified and partially characterised. A cDNA encoding PMT was isolated from an ozone-induced Scots pine cDNA library. Southern blot analysis of the genomic DNA suggested the presence of a gene family. The deduced protein sequence showed the typical highly conserved regions of O-methyltransferases (OMTs), and average identities of 20-56% to known OMTs. PMT expressed in Escherichia coli corresponded to that of purified PMT (40 kDa) from pine cell cultures. The recombinant enzyme catalysed the methylation of PS, caffeic acid, caffeoyl-CoA and quercetin. Several other substances, such as astringenin, resveratrol, 5-OH-ferulic acid, catechol and luteolin, were also methylated. Recombinant PMT thus had a relatively broad substrate specificity. Treatment of 7-year old Scots pine trees with ozone markedly increased the PMT mRNA level. Our results show that PMT represents a new SAM-dependent OMT for the methylation of stress-induced pinosylvin in Scots pine needles.
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