Formic acid decalcification of bone marrow trephines degrades DNA: alternative use of EDTA allows the amplification and sequencing of relatively long PCR products.

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it.

1. Discordance of epidermal growth factor receptor mutation between primary lung tumor and paired distant metastases in non-small cell lung cancer: A systematic review and meta-analysis.PloS one · 2019
   Pooled it
2. Optimizing pathological diagnosis of tuberculosis: qPCR outperforms acid-fast staining in formalin-fixed paraffin-embedded tissues and enables resistance profiling.Frontiers in medicine · 2026
   Article
3. Evaluating the Role of Liquid Biopsy to Detect Pathogenic Homologous Recombination Repair (HRR) Gene Alterations in Metastatic Prostate Cancer.Cancers · 2025
   Article
4. Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow.Veterinary pathology · 2024
   Article
5. Hypertonic saline- and detergent-accelerated EDTA-based decalcification better preserves mRNA of bones.Scientific reports · 2024
   Article
6. Effect of vitamin D deficiency on postorthodontic relapse: An animal study.Clinical and experimental dental research · 2023
   Article
7. Genetic testing for homologous recombination repair (HRR) in metastatic castration-resistant prostate cancer (mCRPC): challenges and solutions.Oncotarget · 2021
   Review
8. The effects of calcitonin on post-orthodontic relapse in rats.Clinical and experimental dental research · 2021
   Article
9. An Ion-exchange Bone Demineralization Method for Improved Time, Expense, and Tissue Preservation.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020
   Article
10. High multiplex analysis of the immune microenvironment in bone marrow trephine samples using GeoMX™ digital spatial profiling.Immuno-oncology technology · 2020
    Article
11. Frequency of GNAS R201H substitution mutation in polyostotic fibrous dysplasia: Pyrosequencing analysis in tissue samples with or without decalcification.Scientific reports · 2017
    Article
12. Influence of decalcification procedures on immunohistochemistry and molecular pathology in breast cancer.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2016
    Article
13. Discordance Between Cobas BRAF V600 Testing and VE1 Immunohistochemistry in a Melanoma Patient With Bone Marrow Metastases.The American Journal of dermatopathology · 2016
    Article
14. Molecular Diagnostics for Precision Medicine in Colorectal Cancer: Current Status and Future Perspective.BioMed research international · 2016
    Review
15. The study of calcified atherosclerotic arteries: an alternative to evaluate the composition of a problematic tissue reveals new insight including metakaryotic cells.BMC clinical pathology · 2016
    Article
16. Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study.Journal of pathology and translational medicine · 2015
    Article
17. Arterial calcification: Finger-pointing at resident and circulating stem cells.World journal of stem cells · 2014
    Review
18. Routine EGFR molecular analysis in non-small-cell lung cancer patients is feasible: exons 18-21 sequencing results of 753 patients and subsequent clinical outcomes.Lung · 2013
    Article
19. Immunohistochemical staining with EGFR mutation-specific antibodies: high specificity as a diagnostic marker for lung adenocarcinoma.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2013
    Article
20. One-step preservation of phosphoproteins and tissue morphology at room temperature for diagnostic and research specimens.PloS one · 2011
    Article

Corrections and comments

• Erratum issued

Authors and funding

6 authors.

Funding

No grant is acknowledged in the PubMed record.

Abstract

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