ArticleBritish journal of pharmacology2001
Pharmacological characterization of muscarinic receptors in dog isolated ciliary and urinary bladder smooth muscle.
Article in British journal of pharmacology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- The Effectiveness of Anticholinergic Therapy for Overactive Bladders: Systematic Review and Meta-Analysis.Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia · 2016Pooled it
- Comparisons of urinary bladder responses to common antimuscarinics reveals unique effects of darifenacin.Frontiers in physiology · 2025Article
- Muscarinic receptors and ligands in cancer.American journal of physiology. Cell physiology · 2009Review
- Muscarinic receptors: their distribution and function in body systems, and the implications for treating overactive bladder.British journal of pharmacology · 2006Review
- Mechanisms mediating cholinergic antral circular smooth muscle contraction in rats.World journal of gastroenterology · 2004Article
- Effects of pirenzepine on pupil size and accommodation in rhesus monkeys.Investigative ophthalmology & visual science · 2004Article
- Acetylcholine induces Ca2+ oscillations via m3/m4 muscarinic receptors in the mouse oocyte.Pflugers Archiv : European journal of physiology · 2003Article
- Gender comparison of muscarinic receptor expression and function in rat and human urinary bladder: differential regulation of M2 and M3 receptors?Naunyn-Schmiedeberg's archives of pharmacology · 2003Article
- M(3) muscarinic receptors mediate contraction of human urinary bladder.British journal of pharmacology · 2002Article
- A benefit-risk assessment of extended-release oxybutynin.Drug safety · 2002Review
- Pharmacological characterization of muscarinic receptors in dog isolated ciliary and urinary bladder smooth muscle.British journal of pharmacology · 2001Article
- The muscarinic M(5) receptor: a silent or emerging subtype?British journal of pharmacology · 2000Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The pharmacological characteristics of muscarinic receptors mediating contraction of dog isolated ciliary muscle were determined and compared to those mediating contraction of dog urinary bladder smooth muscle. 2. (+)-Cis-dioxolane induced concentration-dependent contractions of ciliary muscle (pEC50=7.18+/-0.07, Emax=453+/-64 mg, n=19) and urinary bladder isolated smooth muscle (pEC50=6.55+/-0.07, Emax=11+/-1 g, n=19). These responses were antagonized by several muscarinic receptor antagonists (pKb values for the ciliary muscle and the bladder smooth muscle, respectively): atropine (8.25+/-0.14 and 9.21+/-0.09), pirenzepine (6.31+/-0.13 and 6.70+/-0.25), tolterodine (7.97+/-0.14 and 8.68+/-0.12), oxybutynin (7.40+/-0.08 and 7.88+/-0.12), zamifenacin (6.46+/-0.19 and 7.69+/-0.11), S-secoverine (6.66+/-0.14 and 8.13+/-0.07), AQ-RA 741 (6.16+/-0.15 and 7.08+/-0.23), p-F-HHSiD (7.10+/-0.27 and 7.35+/-0.07) and responses were not antagonized by PD 102807 (up to 100 nM). 3. In urinary bladder smooth muscle, the profile of antagonist pKB values correlated significantly with pK(i) values at human recombinant m3 muscarinic receptors, suggesting that M3 muscarinic receptors mediated the response. In the ciliary muscle, a significant (P<0.01) correlation was obtained with human recombinant m3 and m5 receptors. 4. Darifenacin displayed insurmountable antagonism at receptors in the bladder. At receptors in the ciliary muscle, it exhibited two phases of antagonism, comprising an initial low affinity (pKB<6) component and a high affinity phase (pKB>8). 5. The role of pigmentation in the atypical behaviour of darifenacin was examined. In blue coloured eyes, darifenacin produced apparent surmountable, competitive antagonism of the responses to (+)-cis-dioxolane (pKB=8.76+/-0.07). The antagonist profile obtained in this tissue suggested the involvement of a site which has the pharmacological attributes of the M5 receptor. 6. We suggest that the dog urinary bladder contracts in response to M3 muscarinic receptor activation. Contraction of the brown-eyed dog ciliary muscle is more complex and may include involvement of at least two receptors, possibly the M5 and M3 receptor, whereas blue-eyed dog ciliary muscle may involve a single population of M5 muscarinic receptors.
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