ArticleBritish journal of pharmacology2001
BK channel activation by NS-1619 is partially mediated by intracellular Ca2+ release in smooth muscle cells of porcine coronary artery.
Article in British journal of pharmacology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Lysosomal BK channels facilitate silica-induced inflammation in macrophages.Inhalation toxicology · 2024Article
- Lysosomal Potassium Channels.Handbook of experimental pharmacology · 2023Review
- External Hemin as an Inhibitor of Mitochondrial Large-Conductance Calcium-Activated Potassium Channel Activity.International journal of molecular sciences · 2022Article
- BK Channel in the Physiology and in the Cancer of Pancreatic Duct: Impact and Reliability of BK Openers.Frontiers in pharmacology · 2022Review
- Article
- Two Functional Classes of Rod Bipolar Cells in the Healthy and Degenerated Optogenetically Treated Murine Retina.Frontiers in cellular neuroscience · 2021Article
- A voltage-dependent KThe Journal of cell biology · 2017Article
- Modulation of BK Channel Function by Auxiliary Beta and Gamma Subunits.International review of neurobiology · 2016Review
- Long-term hypoxia increases calcium affinity of BK channels in ovine fetal and adult cerebral artery smooth muscle.American journal of physiology. Heart and circulatory physiology · 2015Article
- Large conductance Ca2+ -activated K+ channel activation with NS1619 decreases myogenic and neurogenic contractions of rat detrusor smooth muscle.European journal of pharmacology · 2011Article
- BK channel activation by NS11021 decreases excitability and contractility of urinary bladder smooth muscle.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Immediate neuronal preconditioning by NS1619.Brain research · 2009Article
- Delayed neuronal preconditioning by NS1619 is independent of calcium activated potassium channels.Journal of neurochemistry · 2008Article
- Functional contribution of the endothelial component to the vasorelaxing effect of resveratrol and NS 1619, activators of the large-conductance calcium-activated potassium channels.Naunyn-Schmiedeberg's archives of pharmacology · 2007Article
- Article
- Functional characterization of large conductance calcium-activated K+ channel openers in bladder and vascular smooth muscle.Naunyn-Schmiedeberg's archives of pharmacology · 2004Article
- BK channel openers inhibit migration of human glioma cells.Pflugers Archiv : European journal of physiology · 2003Article
- Freshly isolated bovine coronary endothelial cells do not express the BK Ca channel gene.The Journal of physiology · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Effects of NS-1619, an opener of large conductance Ca2+-activated K+ (BK) channel, on intracellular Ca2+ concentration ([Ca2+]i) and membrane potential were examined in single myocytes freshly isolated from porcine coronary artery. 2. Under current clamp mode, the application of 1-30 microM NS-1619 hyperpolarized the membrane in concentration-dependent manner. The NS-1619-induced hyperpolarization was abolished by the presence of 100 nM iberiotoxin. 3. Application of 1-10 microM NS-1619 hyperpolarized the membrane by approximately 6 mV or less but did not change significantly the [Ca2+]i. When membrane hyperpolarization of 12 mV or so was caused by 30 microM NS-1619, [Ca2+]i was unexpectedly increased by approximately 200 nM. This increase in [Ca2+]i and the concomitant outward current activation were also observed under voltage-clamp at holding potential of -40 mV. 4. The increase in [Ca2+]i by 30 microM NS-1619 occurred mainly in peripheral regions than in the centre of the myocytes. The removal of extracellular Ca2+ affected neither the membrane hyperpolarization nor the increase in [Ca2+]i. 5. In the presence of 10 mM caffeine and 10 microM ryanodine, the increase in [Ca2+]i by 30 microM NS-1619 was not observed and the membrane hyperpolarization was reduced to approximately 67% of the control. 6. These results indicate that the opening of BK channels by NS-1619 at 30 microM, which is the most frequently used concentration of this agent, is partly due to Ca2+ release from caffeine/ryanodine-sensitive intracellular storage sites but is mainly due to the direct activation of the channels.
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