ArticleAntimicrobial agents and chemotherapy2001
An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tü57.
Article in Antimicrobial agents and chemotherapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Distribution status and influencing factors of antibiotic resistance genes in the Chaohu Lake, China.PeerJ · 2025Article
- Complete sequences of pIJ101-based Streptomyces-Escherichia coli shuttle vectors.Access microbiology · 2024Article
- Optimal Regimens and Clinical Breakpoint of Avilamycin AgainstFrontiers in pharmacology · 2022Article
- Microbial Oligosaccharides with Biomedical Applications.Marine drugs · 2021Review
- Mechanisms of Bacterial Resistance to Antimicrobial Agents.Microbiology spectrum · 2018Article
- Avilamycin and evernimicin induce structural changes in rProteins uL16 and CTC that enhance the inhibition of A-site tRNA binding.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structures of the orthosomycin antibiotics avilamycin and evernimicin in complex with the bacterial 70S ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Avoidance of suicide in antibiotic-producing microbes.Journal of industrial microbiology & biotechnology · 2010Review
- Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on gram-positive bacteria.Clinical microbiology reviews · 2003Review
- Interaction of avilamycin with ribosomes and resistance caused by mutations in 23S rRNA.Antimicrobial agents and chemotherapy · 2002Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
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Abstract
Three different resistance factors from the avilamycin biosynthetic gene cluster of Streptomyces viridochromogenes Tü57, which confer avilamycin resistance when expressed in Streptomyces lividans TK66, were isolated. Analysis of the deduced amino acid sequences showed that AviABC1 is similar to a large family of ATP-binding transporter proteins and that AviABC2 resembles hydrophobic transmembrane proteins known to act jointly with the ATP-binding proteins. The deduced amino acid sequence of aviRb showed similarity to those of other rRNA methyltransferases, and AviRa did not resemble any protein in the databases. Independent expression in S. lividans TK66 of aviABC1 plus aviABC2, aviRa, or aviRb conferred different levels of resistance to avilamycin: 5, 10, or 250 microg/ml, respectively. When either aviRa plus aviRb or aviRa plus aviRb plus aviABC1 plus aviABC2 was coexpressed in S. lividans TK66, avilamycin resistance levels reached more than 250 microg/ml. Avilamycin A inhibited poly(U)-directed polyphenylalanine synthesis in an in vitro system using ribosomes of S. lividans TK66(pUWL201) (GWO), S. lividans TK66(pUWL201-Ra) (GWRa), or S. lividans TK66(pUWL201-Rb) (GWRb), whereas ribosomes of S. lividans TK66 containing pUWL201-Ra+Rb (GWRaRb) were highly resistant. aviRa and aviRb were expressed in Escherichia coli, and both enzymes were purified as fusion proteins to near homogeneity. Both enzymes showed rRNA methyltransferase activity using a mixture of 16S and 23S rRNAs from E. coli as the substrate. Coincubation experiments revealed that the enzymes methylate different positions of rRNA.
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