ArticleMolecular biology of the cell2001
Stathmin/Op18 phosphorylation is regulated by microtubule assembly.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Indolyl-chalcone derivatives trigger apoptosis in cisplatin-resistant mesothelioma cells through aberrant tubulin polymerization and deregulation of microtubule-associated proteins.Frontiers in oncology · 2023Article
- Stathmin Regulates Spatiotemporal Variation in the Memory Loop in Single-Prolonged Stress Rats.Journal of molecular neuroscience : MN · 2020Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration.Cell discovery · 2017Article
- PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.Oncotarget · 2015Article
- Feedback mechanism for microtubule length regulation by stathmin gradients.Biophysical journal · 2014Article
- Pak2 kinase restrains mast cell FcεRI receptor signaling through modulation of Rho protein guanine nucleotide exchange factor (GEF) activity.The Journal of biological chemistry · 2013Article
- Dual detection of chromosomes and microtubules by the chromosomal passenger complex drives spindle assembly.Developmental cell · 2010Article
- Regulation of microtubule dynamics through phosphorylation on stathmin by Epstein-Barr virus kinase BGLF4.The Journal of biological chemistry · 2010Article
- Phosphorylation of alpha6-tubulin by protein kinase Calpha activates motility of human breast cells.The Journal of biological chemistry · 2009Article
- Scaffold mediated regulation of MAPK signaling and cytoskeletal dynamics: a perspective.Cellular signalling · 2007Review
- Chromosomal enrichment and activation of the aurora B pathway are coupled to spatially regulate spindle assembly.Developmental cell · 2007Article
- Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stathmin/Op 18 is a microtubule (MT) dynamics-regulating protein that has been shown to have both catastrophe-promoting and tubulin-sequestering activities. The level of stathmin/Op18 phosphorylation was proved both in vitro and in vivo to be important in modulating its MT-destabilizing activity. To understand the in vivo regulation of stathmin/Op18 activity, we investigated whether MT assembly itself could control phosphorylation of stathmin/Op18 and thus its MT-destabilizing activity. We found that MT nucleation by centrosomes from Xenopus sperm or somatic cells and MT assembly promoted by dimethyl sulfoxide or paclitaxel induced stathmin/Op18 hyperphosphorylation in Xenopus egg extracts, leading to new stathmin/Op18 isoforms phosphorylated on Ser 16. The MT-dependent phosphorylation of stathmin/Op18 took place in interphase extracts as well, and was also observed in somatic cells. We show that the MT-dependent phosphorylation of stathmin/Op18 on Ser 16 is mediated by an activity associated to the MTs, and that it is responsible for the stathmin/Op18 hyperphosphorylation reported to be induced by the addition of "mitotic chromatin." Our results suggest the existence of a positive feedback loop, which could represent a novel mechanism contributing to MT network control.
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