ArticleMolecular biology of the cell2001
Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- RNA localization to nuclear speckles follows splicing logic.Nucleic acids research · 2026Article
- Article
- Viral-host interactions during splicing and nuclear export of influenza virus mRNAs.Current opinion in virology · 2022Review
- Function of Cajal Bodies in Nuclear RNA Retention inInternational journal of molecular sciences · 2022Article
- Nuclear speckles - a driving force in gene expression.Journal of cell science · 2022Review
- Nuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention.Nature communications · 2021Article
- Nuclear mechanisms of gene expression control: pre-mRNA splicing as a life or death decision.Current opinion in genetics & development · 2021Review
- Splicing at the phase-separated nuclear speckle interface: a model.Nucleic acids research · 2021Review
- Chemical intervention of influenza virus mRNA nuclear export.PLoS pathogens · 2020Article
- Reorganization of the nuclear compartments involved in transcription and RNA processing in myonuclei of type I spinal muscular atrophy.Histochemistry and cell biology · 2019Article
- Uncoupling of nucleo-cytoplasmic RNA export and localization during stress.Nucleic acids research · 2019Article
- Genome organization around nuclear speckles.Current opinion in genetics & development · 2019Review
- Intronless mRNAs transit through nuclear speckles to gain export competence.The Journal of cell biology · 2018Article
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- Influenza virus mRNA trafficking through host nuclear speckles.Nature microbiology · 2016Article
- Detained introns are a novel, widespread class of post-transcriptionally spliced introns.Genes & development · 2015Article
- NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins.Nucleic acids research · 2014Article
- Article
- Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion.Nature communications · 2012Article
- A role for TREX components in the release of spliced mRNA from nuclear speckle domains.Nature communications · 2010Article
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5 authors.
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Abstract
Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjection of antisense deoxyoligoribonucleotides complementary to the specific domains of snRNAs. Under these latter conditions prespliceosomal complexes are formed on endogenous pre-mRNAs. We conclude that the (pre)spliceosomal complexes on microinjected pre-mRNA are formed inside the speckles. Their targeting into and accumulation in the speckles is a result of the cumulative loading of splicing factors to the pre-mRNA and the complexes formed give rise to the speckled pattern observed.
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