ArticleMolecular biology of the cell2001
Phosphorylation of a conserved integrin alpha 3 QPSXXE motif regulates signaling, motility, and cytoskeletal engagement.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- KRAS Addiction Promotes Cancer Cell Adaptation in Harsh Microenvironment Through Macropinocytosis.Sub-cellular biochemistry · 2022Article
- β2 Integrin CD11d/CD18: From Expression to an Emerging Role in Staged Leukocyte Migration.Frontiers in immunology · 2021Review
- Quantitative phosphoproteomic analysis identifies the potential therapeutic target EphA2 for overcoming sorafenib resistance in hepatocellular carcinoma cells.Experimental & molecular medicine · 2020Article
- Tetraspanin CD9: A Key Regulator of Cell Adhesion in the Immune System.Frontiers in immunology · 2018Review
- Tetraspanins as regulators of the tumour microenvironment: implications for metastasis and therapeutic strategies.British journal of pharmacology · 2014Review
- CD81 regulates cell migration through its association with Rac GTPase.Molecular biology of the cell · 2013Article
- Article
- Loss of adenomatous poliposis coli-α3 integrin interaction promotes endothelial apoptosis in mice and humans.Circulation research · 2012Article
- Tetraspanin CD82 inhibits protrusion and retraction in cell movement by attenuating the plasma membrane-dependent actin organization.PloS one · 2012Article
- Tetraspanin CO-029 inhibits colorectal cancer cell movement by deregulating cell-matrix and cell-cell adhesions.PloS one · 2012Article
- The C-terminal tail of tetraspanin protein CD9 contributes to its function and molecular organization.Journal of cell science · 2011Article
- Functional and biochemical studies of CD9 in fibrosarcoma cell line.Molecular and cellular biochemistry · 2011Article
- Glycoprotein capture and quantitative phosphoproteomics indicate coordinated regulation of cell migration upon lysophosphatidic acid stimulation.Molecular & cellular proteomics : MCP · 2010Article
- Discovery and characterization of a small molecule inhibitor of the PDZ domain of dishevelled.The Journal of biological chemistry · 2009Article
- Transmembrane interactions are needed for KAI1/CD82-mediated suppression of cancer invasion and metastasis.The American journal of pathology · 2009Article
- A critical role for tetraspanin CD151 in alpha3beta1 and alpha6beta4 integrin-dependent tumor cell functions on laminin-5.Molecular biology of the cell · 2006Article
- Physiological and pathological roles of alpha3beta1 integrin.The Journal of membrane biology · 2004Review
- Dynamic regulation of a GPCR-tetraspanin-G protein complex on intact cells: central role of CD81 in facilitating GPR56-Galpha q/11 association.Molecular biology of the cell · 2004Article
- Spatial restriction of alpha4 integrin phosphorylation regulates lamellipodial stability and alpha4beta1-dependent cell migration.The Journal of cell biology · 2003Article
- Comparison of the mechanisms of nongenomic actions of thyroid hormone and steroid hormones.Journal of endocrinological investigation · 2002Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Integrin alpha 3A cytoplasmic tail phosphorylation was mapped to amino acid S1042, as determined by mass spectrometry, and confirmed by mutagenesis. This residue occurs within a "QPSXXE" motif conserved in multiple alpha chains (alpha 3A, alpha 6A, alpha 7A), from multiple species. Phosphorylation of alpha 3A and alpha 6A did not appear to be directly mediated by protein kinase C (PKC) alpha, beta, gamma, delta, epsilon, zeta, or mu, or by any of several other known serine kinases, although PKC has an indirect role in promoting phosphorylation. A S1042A mutation did not affect alpha 3-Chinese hamster ovary (CHO) cell adhesion to laminin-5, but did alter 1) alpha 3-dependent tyrosine phosphorylation of focal adhesion kinase and paxillin (in the presence or absence of phorbol 12-myristate 13 acetate stimulation), and p130(CAS) (in the absence of phorbol 12-myristate 13 acetate stimulation), 2) the shape of cells spread on laminin-5, and 3) alpha 3-dependent random CHO cell migration on laminin-5. In addition, S1042A mutation altered the PKC-dependent, ligand-dependent subcellular distribution of alpha 3 and F-actin in CHO cells. Together, the results demonstrate clearly that alpha 3A phosphorylation is functionally relevant. In addition, the results strongly suggest that alpha 3 phosphorylation may regulate alpha 3 integrin interaction with the cytoskeleton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.