ArticleMolecular biology of the cell2001
Cyclic AMP increases cell surface expression of functional Na,K-ATPase units in mammalian cortical collecting duct principal cells.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 73 citations in OpenAlex.
- Alterations in renal Na,K-ATPase activity and protein expression in rat models of pressure and volume overload.Scientific reports · 2025Article
- Localization of the Neuropeptide Arginine Vasotocin and Its Receptor in the Osmoregulatory Organs of Black Porgy,International journal of molecular sciences · 2022Article
- Emerging therapies for autosomal dominant polycystic kidney disease with a focus on cAMP signaling.Frontiers in molecular biosciences · 2022Review
- Dietary sodium induces a redistribution of the tubular metabolic workload.The Journal of physiology · 2017Article
- Systems-level identification of PKA-dependent signaling in epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Identification of anti-viral activity of the cardenolides, NaToxicology and applied pharmacology · 2017Article
- cAMP-dependent regulation ofThe Journal of general physiology · 2017Article
- Coordinated Control of ENaC and Na+,K+-ATPase in Renal Collecting Duct.Journal of the American Society of Nephrology : JASN · 2016Review
- The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na(+)/K(+)-ATPase Activity of Culex quinquefasciatus Larvae.The Journal of membrane biology · 2016Article
- The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site.The Biochemical journal · 2015Article
- Evidence for cyclooxygenase-dependent sweating in young males during intermittent exercise in the heat.The Journal of physiology · 2014Article
- MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly.Molecular biology of the cell · 2014Article
- Sodium transport is modulated by p38 kinase-dependent cross-talk between ENaC and Na,K-ATPase in collecting duct principal cells.Journal of the American Society of Nephrology : JASN · 2014Article
- Adenylyl cyclase VI mediates vasopressin-stimulated ENaC activity.Journal of the American Society of Nephrology : JASN · 2013Article
- Cyclic stretch stimulates recruitment of active Na⁺/K⁺-ATPase subunits to the plasma membrane of skeletal muscle cells.Molecular and cellular biochemistry · 2012Article
- Collecting duct-specific knockout of adenylyl cyclase type VI causes a urinary concentration defect in mice.American journal of physiology. Renal physiology · 2012Article
- Characterization of vasopressin-responsive collecting duct adenylyl cyclases in the mouse.American journal of physiology. Renal physiology · 2010Article
- Regulation of epithelial Na+ transport by soluble adenylyl cyclase in kidney collecting duct cells.The Journal of biological chemistry · 2009Article
- Regulation of NaCl transport in the renal collecting duct: lessons from cultured cells.Pflugers Archiv : European journal of physiology · 2006Review
- Molecular identification of Sch28080-sensitive K-ATPase activities in the mouse kidney.Pflugers Archiv : European journal of physiology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclic AMP (cAMP) stimulates the transport of Na(+) and Na,K-ATPase activity in the renal cortical collecting duct (CCD). The aim of this study was to investigate the mechanism whereby cAMP stimulates the Na,K-ATPase activity in microdissected rat CCDs and cultured mouse mpkCCD(c14) collecting duct cells. db-cAMP (10(-3) M) stimulated by 2-fold the activity of Na,K-ATPase from rat CCDs as well as the ouabain-sensitive component of (86)Rb(+) uptake by rat CCDs (1.7-fold) and cultured mouse CCD cells (1.5-fold). Pretreatment of rat CCDs with saponin increased the total Na,K-ATPase activity without further stimulation by db-cAMP. Western blotting performed after a biotinylation procedure revealed that db-cAMP increased the amount of Na,K-ATPase at the cell surface in both intact rat CCDs (1.7-fold) and cultured cells (1.3-fold), and that this increase was not related to changes in Na,K-ATPase internalization. Brefeldin A and low temperature (20 degrees C) prevented both the db-cAMP-dependent increase in cell surface expression and activity of Na,K-ATPase in both intact rat CCDs and cultured cells. Pretreatment with the intracellular Ca(2+) chelator bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid also blunted the increment in cell surface expression and activity of Na,K-ATPase caused by db-cAMP. In conclusion, these results strongly suggest that the cAMP-dependent stimulation of Na,K-ATPase activity in CCD results from the translocation of active pump units from an intracellular compartment to the plasma membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.