ArticleThe Journal of physiology2001
Intracellular pathways regulating ciliary beating of rat brain ependymal cells.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 98 citations in OpenAlex.
- Multiciliated ependymal cells: an update on biology and pathology in the adult brain.Acta neuropathologica · 2024Review
- High-Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Roles of Ependymal Cells in the Physiology and Pathology of the Central Nervous System.Aging and disease · 2023Review
- History of research concerning the ependyma: a view from inside the human brain.Frontiers in cellular neuroscience · 2023Review
- All the brain's a stage for serotonin: the forgotten story of serotonin diffusion across cell membranes.Proceedings. Biological sciences · 2022Review
- Secondary Brain Injury Following Neonatal Intraventricular Hemorrhage: The Role of the Ciliated Ependyma.Frontiers in pediatrics · 2022Review
- Neuronal coordination of motile cilia in locomotion and feeding.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- The development and functions of multiciliated epithelia.Nature reviews. Molecular cell biology · 2017Review
- Planar Organization of Multiciliated Ependymal (E1) Cells in the Brain Ventricular Epithelium.Trends in neurosciences · 2016Review
- Article
- CSF flow pathways through the ventricle-cistern interfaces in kaolin-induced hydrocephalus rats-laboratory investigation.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2015Article
- Three types of ependymal cells with intracellular calcium oscillation are characterized by distinct cilia beating properties.Journal of neuroscience research · 2014Article
- Axonal control of the adult neural stem cell niche.Cell stem cell · 2014Article
- Structure and function of the ependymal barrier and diseases associated with ependyma disruption.Tissue barriers · 2014Review
- Axons take a dive: Specialized contacts of serotonergic axons with cells in the walls of the lateral ventricles in mice and humans.Neurogenesis (Austin, Tex.) · 2014Article
- Neuronal control of pedal sole cilia in the pond snail Lymnaea stagnalis appressa.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2013Article
- Mechanisms of hydrocephalus after neonatal and adult intraventricular hemorrhage.Translational stroke research · 2012Article
- Stress-related serotonergic systems: implications for symptomatology of anxiety and affective disorders.Cellular and molecular neurobiology · 2012Review
- Functional topography of midbrain and pontine serotonergic systems: implications for synaptic regulation of serotonergic circuits.Psychopharmacology · 2011Review
- Mucin secretion induced by titanium dioxide nanoparticles.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The mammalian brain ventricles are lined with ciliated ependymal cells. As yet little is known about the mechanisms by which neurotransmitters regulate cilia beat frequency (CBF). 2. Application of 5-HT to ependymal cells in cultured rat brainstem slices caused CBF to increase. 5-HT had an EC50 of 30 microM and at 100 microM attained a near-maximal CBF increase of 52.7 +/- 4.1 % (mean +/- s.d.) (n = 8). 3. Bathing slices in Ca2+-free solution markedly reduced the 5-HT-mediated increase in CBF. Fluorescence measurements revealed that 5-HT caused a marked transient elevation in cytosolic Ca2+ ([Ca2+]c) that then slowly decreased to a plateau level. Analysis showed that the [Ca2+]c transient was due to release of Ca2+ from inositol 1,4,5-trisphosphate (IP3)-sensitive stores; the plateau was probably due to extracellular Ca2+ influx through Ca2+ release-activated Ca2+ (CRAC) channels. 4. Application of ATP caused a sustained decrease in CBF. ATP had an EC50 of about 50 microM and 100 microM ATP resulted in a maximal 57.5 +/- 6.5 % (n = 12) decrease in CBF. The ATP-induced decrease in CBF was unaffected by lowering extracellular [Ca2+], and no changes in [Ca2+]c were observed. Exposure of ependymal cells to forskolin caused a decrease in CBF. Ciliated ependymal cells loaded with caged cAMP exhibited a 54.3 +/- 7.5 % (n = 9) decrease in CBF following uncaging. These results suggest that ATP reduces CBF by a Ca2+-independent cAMP-mediated pathway. 5. Application of 5-HT and adenosine-5'-O-3-thiotriphosphate (ATP-gamma-S) to acutely isolated ciliated ependymal cells resulted in CBF responses similar to those of ependymal cells in cultured slices suggesting that these neurotransmitters act directly on these cells. 6. The opposite response of ciliated ependymal cells to 5-HT and ATP provides a novel mechanism for their active involvement in central nervous system signalling.
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