ArticleInfection and immunity2001
Acid-induced gene expression in Helicobacter pylori: study in genomic scale by microarray.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 129 citations in OpenAlex.
- Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System.Infection and immunity · 2021Article
- Acid-regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization.Helicobacter · 2018Article
- Dissecting the Acid Stress Response ofFrontiers in microbiology · 2018Article
- Survival of Helicobacter pylori in gastric acidic territory.Helicobacter · 2017Review
- Gene Expression Profiling of Transcription Factors ofFrontiers in microbiology · 2017Article
- Multipronged regulatory functions of a novel endonuclease (TieA) from Helicobacter pylori.Nucleic acids research · 2016Article
- Article
- Expression of the Helicobacter pylori virulence factor vacuolating cytotoxin A (vacA) is influenced by a potential stem-loop structure in the 5' untranslated region of the transcript.Molecular microbiology · 2015Article
- Identification of PblB mediating galactose-specific adhesion in a successful Streptococcus pneumoniae clone.Scientific reports · 2015Article
- Gastric acid-dependent diseases: a twentieth-century revolution.Digestive diseases and sciences · 2014Article
- A second-generation protein-protein interaction network of Helicobacter pylori.Molecular & cellular proteomics : MCP · 2014Article
- Clinical proteomics identifies potential biomarkers in Helicobacter pylori for gastrointestinal diseases.World journal of gastroenterology · 2014Review
- In vivo expression of Helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer.Infection and immunity · 2012Article
- Functional analysis of an acid adaptive DNA adenine methyltransferase from Helicobacter pylori 26695.PloS one · 2011Article
- Ulcerogenic Helicobacter pylori strains isolated from children: a contribution to get insight into the virulence of the bacteria.PloS one · 2011Article
- Coupled amino acid deamidase-transport systems essential for Helicobacter pylori colonization.Infection and immunity · 2010Article
- Role of GlnR in acid-mediated repression of genes encoding proteins involved in glutamine and glutamate metabolism in Streptococcus mutans.Applied and environmental microbiology · 2010Article
- Gastric infection by Helicobacter pylori.Current gastroenterology reports · 2009Review
- Response of gastric epithelial progenitors to Helicobacter pylori Isolates obtained from Swedish patients with chronic atrophic gastritis.The Journal of biological chemistry · 2009Article
- Synergistic effect of imp/ostA and msbA in hydrophobic drug resistance of Helicobacter pylori.BMC microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the RNA expression in response to acid stress of Helicobacter pylori in genomic scale, a microarray membrane containing 1,534 open reading frames (ORFs) from strain 26695 was used. Total RNAs of H. pylori under growth conditions of pH 7.2 and 5.5 were extracted, reverse transcribed into cDNA, and labeled with biotin. Each microarray membrane was hybridized with cDNA probe from the same strain under two different pH conditions and developed by a catalyzed reporter deposition method. Gene expression of all ORFs was measured by densitometry. Among the 1,534 ORFs, 53 ORFs were highly expressed (> or = 30% of rRNA control in densitometry ratios). There were 445 ORFs which were stably expressed (<30% of rRNA in densitometry) under both pH conditions without significant variation. A total of 80 ORFs had significantly increased expression levels at low pH, while expressions of 4 ORFs were suppressed under acidic condition. The remaining 952 ORFs were not detectable under either pH condition. These data were highly reproducible and comparable to those obtained by the RNA slot blot method. Our results suggest that microarray can be used in monitoring prokaryotic gene expression in genomic scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.