ArticleThe EMBO journal2001
Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 122 citations in OpenAlex.
- Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme.Nature communications · 2026Article
- Plant mitochondrial PORR proteins facilitate group II intron splicing by binding to distinct regions within their target introns.Nucleic acids research · 2025Article
- Trypanosoma cruzi has Two Peptidyl-tRNA Hydrolases Showing Different Localization and Function.Acta parasitologica · 2025Article
- Pentatricopeptide repeat proteins in plants: Cellular functions, action mechanisms, and potential applications.Plant communications · 2025Review
- GhCTSF1, a short PPR protein with a conserved role in chloroplast development and photosynthesis, participates in intron splicing of rpoC1 and ycf3-2 transcripts in cotton.Plant communications · 2024Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- A Single Nucleotide Variation ofInternational journal of molecular sciences · 2023Article
- Updated Progress on Group II Intron Splicing Factors in Plant Chloroplasts.Current issues in molecular biology · 2022Review
- An overview of RNA splicing and functioning of splicing factors in land plant chloroplasts.RNA biology · 2022Article
- Functions of PPR Proteins in Plant Growth and Development.International journal of molecular sciences · 2021Review
- A RanBP2-type zinc finger protein functions in intron splicing in Arabidopsis mitochondria and is involved in the biogenesis of respiratory complex I.Nucleic acids research · 2021Article
- Arabidopsis Mitochondrial Transcription Termination Factor mTERF2 Promotes Splicing of Group IIB Introns.Cells · 2021Article
- The ChloroplastCells · 2021Review
- Arabidopsis mTERF9 protein promotes chloroplast ribosomal assembly and translation by establishing ribonucleoprotein interactions in vivo.Nucleic acids research · 2021Article
- Rerouting of ribosomal proteins into splicing in plant organelles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice.BMC plant biology · 2020Article
- The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis inPlants (Basel, Switzerland) · 2020Article
- PPR14 Interacts With PPR-SMR1 and CRM Protein Zm-mCSF1 to Facilitate Mitochondrial Intron Splicing in Maize.Frontiers in plant science · 2020Article
- An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.The Plant cell · 2019Article
- The DEAD-box RNA Helicase RH50 Is a 23S-4.5S rRNA Maturation Factor that Functionally Overlaps with the Plastid Signaling Factor GUN1.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Group II introns are catalytic RNAs that have been proposed to be the evolutionary precursors to the spliceosome. Most group II introns require accessory factors to splice efficiently in vivo, but few such factors have been identified. We have cloned the maize nuclear gene crs2, which is required for the splicing of nine group II introns in chloroplasts. CRS2 is related to peptidyl-tRNA hydrolase enzymes. However, CRS2 expression failed to rescue an Escherichia coli pth(ts) mutant and CRS2 lacks several conserved amino acids that are important for the activity of the E.coli enzyme, indicating that it may lack peptidyl-tRNA hydrolase activity. CRS2 is localized to the chloroplast stroma, where it is found in a large salt-stable complex that contains RNA. CRS2 co-sediments with group II intron RNA during centrifugation of stroma through sucrose gradients, suggesting that CRS2 facilitates splicing via direct interaction with intron RNA. Sequence comparisons indicate how evolutionary tinkering may have allowed an enzyme that interacts with peptidyl-tRNAs to acquire a function in group II intron splicing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.