ArticleThe EMBO journal2001
Gal80-Gal80 interaction on adjacent Gal4p binding sites is required for complete GAL gene repression.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
- Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor.Nature communications · 2023Article
- A double role of the Gal80 N terminus in activation of transcription by Gal4p.Life science alliance · 2020Article
- Monitoring of switches in heterochromatin-induced silencing shows incomplete establishment and developmental instabilities.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Polymorphisms in the yeast galactose sensor underlie a natural continuum of nutrient-decision phenotypes.PLoS genetics · 2017Article
- Design and Construction of Generalizable RNA-Protein Hybrid Controllers by Level-Matched Genetic Signal Amplification.Cell systems · 2016Article
- Hsp90 Maintains Proteostasis of the Galactose Utilization Pathway To Prevent Cell Lethality.Molecular and cellular biology · 2016Article
- Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.Molecular and cellular biology · 2013Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation.The Journal of biological chemistry · 2013Article
- Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution.Nature communications · 2012Article
- Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80.Genetics · 2011Article
- Modeling the evolution of a classic genetic switch.BMC systems biology · 2011Article
- A general mechanism for network-dosage compensation in gene circuits.Science (New York, N.Y.) · 2010Article
- Epigenetics of the yeast galactose genetic switch.Journal of biosciences · 2009Review
- Combinatorial promoter design for engineering noisy gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Desiccation and zinc binding induce transition of tomato abscisic acid stress ripening 1, a water stress- and salt stress-regulated plant-specific protein, from unfolded to folded state.Plant physiology · 2007Article
- Scanning sequences after Gibbs sampling to find multiple occurrences of functional elements.BMC bioinformatics · 2006Article
- Replacement of a conserved tyrosine by tryptophan in Gal3p of Saccharomyces cerevisiae reduces constitutive activity: implications for signal transduction in the GAL regulon.Molecular genetics and genomics : MGG · 2005Article
- Mutational hypersensitivity of a gene regulatory protein: Saccharomyces cerevisiae Gal80p.Genetics · 2005Article
- Steady-state analysis of glucose repression reveals hierarchical expression of proteins under Mig1p control in Saccharomyces cerevisiae.The Biochemical journal · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Regulation of the GAL genes of Saccharomyces cerevisiae is determined by the interplay of the transcriptional activator Gal4p and the repressor Gal80p, which binds and masks the activation domain of Gal4p under non-inducing conditions. Here we demonstrate that Gal80p dimerizes with high affinity and that this dimerization appears to stabilize the Gal4p-Gal80p interaction and also, indirectly, the Gal4p-DNA interaction in a (Gal4p)2(Gal80p)2DNA complex. In addition, Gal80 dimers transiently interact with each other to form higher order multimers. We provide evidence that adjacent Gal4p binding sites, when correctly spaced, greatly stabilize Gal80p dimer-dimer interactions and that this stabilization results in the complete repression of GAL genes with multiple Gal4p binding sites. In contrast, GAL genes under the control of a single Gal4p binding site do not stabilize Gal80p multimers, resulting in significant and biologically important transcriptional leakage. Cooperative binding experiments indicate that Gal80p dimer-dimer interaction probably does not lead to a stronger Gal4p-Gal80p interaction, but most likely to a more complete shielding of the Gal4p activation domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.