ArticleGene therapy2000
Long-term gene transfer to mouse fetuses with recombinant adenovirus and adeno-associated virus (AAV) vectors.
Article in Gene therapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- Introducing PIGMO, a novel PIGmented MOuse model of Parkinson's disease.NPJ Parkinson's disease · 2026Article
- p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.Cancer cell · 2019Article
- Brain cytoplasmic RNA 1 suppresses smooth muscle differentiation and vascular development in mice.The Journal of biological chemistry · 2018Article
- Augmentation of transgene-encoded protein after neonatal injection of adeno-associated virus improves hepatic copy number without immune responses.Pediatric research · 2015Article
- Intra-amniotic rAAV-mediated microdystrophin gene transfer improves canine X-linked muscular dystrophy and may induce immune tolerance.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- In utero lung gene transfer using adeno-associated viral and lentiviral vectors in mice.Human gene therapy methods · 2014Article
- Kidney-specific expression of GFP by in-utero delivery of pseudotyped adeno-associated virus 9.Molecular therapy. Methods & clinical development · 2014Article
- A novel surgical approach for intratracheal administration of bioactive agents in a fetal mouse model.Journal of visualized experiments : JoVE · 2012Article
- The fetal respiratory system as target for antenatal therapy.Facts, views & vision in ObGyn · 2011Review
- Efficient gene transfer into the mouse lung by fetal intratracheal injection of rAAV2/6.2.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- The developmental stage determines the distribution and duration of gene expression after early intra-amniotic gene transfer using lentiviral vectors.Gene therapy · 2010Article
- Systemic delivery of AAV8 in utero results in gene expression in diaphragm and limb muscle: treatment implications for muscle disorders.Gene therapy · 2009Article
- Efficient in vivo targeting of epidermal stem cells by early gestational intraamniotic injection of lentiviral vector driven by the keratin 5 promoter.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
- Evaluation of prenatal intra-amniotic LAMB3 gene delivery in a mouse model of Herlitz disease.Gene therapy · 2006Article
- Hepatocyte growth factor receptor is a coreceptor for adeno-associated virus type 2 infection.Journal of virology · 2005Article
- Comparison of high-capacity and first-generation adenoviral vector gene delivery to murine muscle in utero.Gene therapy · 2005Article
- Recombinant adeno-associated viral (rAAV) vectors as therapeutic tools for Duchenne muscular dystrophy (DMD).Gene therapy · 2004Review
- Fetal muscle gene transfer is not enhanced by an RGD capsid modification to high-capacity adenoviral vectors.Gene therapy · 2003Article
- In utero recombinant adeno-associated virus gene transfer in mice, rats, and primates.BMC biotechnology · 2003Article
- Modulation of hepatic granulomatous responses by transgene expression of DAP12 or TREM-1-Ig molecules.The American journal of pathology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We have developed a micro-injection technique to deliver recombinant adenovirus and AAV to mouse fetuses at day 15 after conception. Several routes of delivery, including injections to the amniotic fluid, the front limb, the placenta, the liver, and the retro-orbital venus plexus, were tested using an E1-deleted recombinant adenovirus (Ad.CBlacZ) or a recombinant adeno-associated virus (AAV.CMVlacZ) carrying a beta-galactosidase (lacZ) gene. Injection of Ad.CBlacZ into the amniotic cavity led to transgene expression in the skin and in the digestive tract of the fetuses. Injection of Ad.CBlacZ in the front limb resulted in LacZ expression in all major muscle groups around the injection site and at low levels in the liver. The other three routes of delivery, ie intra-placental, intra-hepatic and retro-orbital injections of Ad.CBlacZ, all led to lacZ expression predominantly in the liver. Further studies revealed a maximal tolerant dose (defined as the highest viral dose with < or =20% mortality in the injected fetuses) of 1 x 10(9) particles per fetus for intra- hepatic injections, 3 x 10(9) particles per fetus for intra-placental injection, 1 x 1010 particles per fetus for retro-orbital and intra-amniotic injections, and 2 x 10(10) particle per fetus for intra-muscular injection. The adenovirus-mediated lacZ expression in liver and muscle persisted for at least 6 weeks. Intra-muscular injection of AAV.CMVlacZ also resulted in lacZ expression in the muscle up to 3 months after birth with no indication of cellular immune response at the injection site. Taken together, our results demonstrated that prolonged transgene expression can be achieved by in utero gene transfer using either adenoviral or AAV vectors. The distribution of virus-mediated gene transfer appeared to determined mostly by the route of viral administration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.