ArticleProceedings of the National Academy of Sciences of the United States of America2001
Genetic transformation of HeLa cells by Agrobacterium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 300 citations in OpenAlex.
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Type IV secretion systems: reconciling diversity through a unified nomenclature.FEMS microbiology reviews · 2026Review
- Binary vector copy number engineering improves Agrobacterium-mediated transformation.Nature biotechnology · 2025Article
- Screening conditions and constructs for attempted genetic transformation of C. elegans by Agrobacterium.PloS one · 2025Article
- Article
- DNA Transport through the Dynamic Type IV Secretion System.Infection and immunity · 2023Review
- A modified Agrobacterium-mediated transformation for two oomycete pathogens.PLoS pathogens · 2023Article
- T-LOC: A comprehensive tool to localize and characterize T-DNA integration sites.Plant physiology · 2022Article
- Oxicam-type nonsteroidal anti-inflammatory drugs enhancePlant biotechnology (Tokyo, Japan) · 2022Article
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Gut microbial differences in breast and prostate cancer cases from two randomised controlled trials compared to matched cancer-free controls.Beneficial microbes · 2021Article
- Review
- Genetic transformation ofeLife · 2020Article
- Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2.BioImpacts : BI · 2020Article
- Trans-Kingdom Conjugation within Solid Media fromInternational journal of molecular sciences · 2019Article
- Stable pH Suppresses Defense Signaling and is the Key to Enhance Agrobacterium-Mediated Transient Expression in Arabidopsis Seedlings.Scientific reports · 2018Article
- The Agrobacterium VirE2 effector interacts with multiple members of the Arabidopsis VIP1 protein family.Molecular plant pathology · 2018Article
- Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis.BMC microbiology · 2018Article
- The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome.Applied and environmental microbiology · 2017Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens is a soil phytopathogen that elicits neoplastic growths on the host plant species. In nature, however, Agrobacterium also may encounter organisms belonging to other kingdoms such as insects and animals that feed on the infected plants. Can Agrobacterium, then, also infect animal cells? Here, we report that Agrobacterium attaches to and genetically transforms several types of human cells. In stably transformed HeLa cells, the integration event occurred at the right border of the tumor-inducing plasmid's transferred-DNA (T-DNA), suggesting bona fide T-DNA transfer and lending support to the notion that Agrobacterium transforms human cells by a mechanism similar to that which it uses for transformation of plants cells. Collectively, our results suggest that Agrobacterium can transport its T-DNA to human cells and integrate it into their genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.