ArticleProceedings of the National Academy of Sciences of the United States of America2001
Real-time visualization of intracellular hydrodynamics in single living cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 182 citations in OpenAlex.
- Inertial sensing of water content in tumor spheroids.Science advances · 2026Article
- The cytoplasm of living cells can sustain transient and steady intracellular pressure gradients.eLife · 2026Article
- Envisioning the Future of the Neurosurgical Operating Room with the Concept of the Medical Metaverse.Journal of Korean Neurosurgical Society · 2025Article
- Uses of coherent Raman scattering microscopy in neuroscience.Frontiers in neuroscience · 2025Review
- Imaging Metabolic Flow of Water in Plants with Isotope-Traced Stimulated Raman Scattering Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Neumann's principle based eigenvector approach for deriving non-vanishing tensor elements for nonlinear optics.The Journal of chemical physics · 2022Article
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- Assessment of the antimicrobial effects of sodium hypochlorite onHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2022Article
- Research Progress in the Medical Application of Heavy Water, Especially in the Field of DInternational journal of medical sciences · 2022Review
- Poroelastic osmoregulation of living cell volume.iScience · 2021Article
- Coherent Raman scattering microscopy: capable solution in search of a larger audience.Journal of biomedical optics · 2021Article
- Non-Uniform Distribution of Cryoprotecting Agents in Rice Culture Cells Measured by CARS Microscopy.Plants (Basel, Switzerland) · 2021Article
- Article
- Rapid Determination of Antimicrobial Susceptibility by Stimulated Raman Scattering Imaging of DAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Rise of Raman spectroscopy in neurosurgery: a review.Journal of biomedical optics · 2020Review
- Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy.Nature communications · 2019Article
- Characterizing stratum corneum structure, barrier function, and chemical content of human skin with coherent Raman scattering imaging.Biomedical optics express · 2018Article
- Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source.Biomedical optics express · 2018Article
- Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Growth is required for perception of water availability to pattern root branches in plants.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high spatial resolution. Studies of the hydrodynamics in the microorganism Dictyostelium discoideum indicated the presence of a microscopic region near the plasma membrane where the mobility of water molecules is severely restricted. Modeling the transient hydrodynamics eventuated in the determination of cell-specific cytosolic diffusion and plasma membrane permeability constants. Our experiments demonstrate that CARS microscopy offers an invaluable tool for probing single-cell water dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.