ArticleProceedings of the National Academy of Sciences of the United States of America2001
Electrophysiological characterization of specific interactions between bacterial sensory rhodopsins and their transducers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
- Convergent evolution of animal and microbial rhodopsins.RSC advances · 2023Review
- Initial photophysical characterization of the proteorhodopsin optical proton sensor (PROPS).Frontiers in neuroscience · 2015Article
- Mechanism divergence in microbial rhodopsins.Biochimica et biophysica acta · 2014Review
- From channelrhodopsins to optogenetics.EMBO molecular medicine · 2013Article
- Voltage dependence of proton pumping by bacteriorhodopsin mutants with altered lifetime of the M intermediate.PloS one · 2013Article
- Opposite displacement of helix F in attractant and repellent signaling by sensory rhodopsin-Htr complexes.The Journal of biological chemistry · 2011Article
- Sensory rhodopsin-I as a bidirectional switch: opposite conformational changes from the same photoisomerization.Biophysical journal · 2011Article
- A microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties.The Journal of biological chemistry · 2011Article
- The photochemical reaction cycle and photoinduced proton transfer of sensory rhodopsin II (Phoborhodopsin) from Halobacterium salinarum.Biophysical journal · 2010Article
- Phototactic and chemotactic signal transduction by transmembrane receptors and transducers in microorganisms.Sensors (Basel, Switzerland) · 2010Review
- Reaction dynamics of halorhodopsin studied by time-resolved diffusion.Biophysical journal · 2009Article
- A Schiff base connectivity switch in sensory rhodopsin signaling.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Time resolved kinetics of the guinea pig Na-Ca exchanger (NCX1) expressed in Xenopus oocytes: voltage and Ca(2+) dependence of pre-steady-state current investigated by photolytic Ca (2+)concentration jumps.Pflugers Archiv : European journal of physiology · 2007Article
- Three strategically placed hydrogen-bonding residues convert a proton pump into a sensory receptor.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Role of Arg-72 of pharaonis Phoborhodopsin (sensory rhodopsin II) on its photochemistry.Biophysical journal · 2004Article
- Channelrhodopsin-2, a directly light-gated cation-selective membrane channel.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Electric-field dependent decays of two spectroscopically different M-states of photosensory rhodopsin II from Natronobacterium pharaonis.Biophysical journal · 2003Article
- FTIR spectroscopy of the M photointermediate in pharaonis rhoborhodopsin.Biophysical journal · 2002Article
- Role of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II).Biophysical journal · 2002Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The halobacterial phototaxis receptors sensory rhodopsin I and II (SRI, SRII) enable the bacteria to seek optimal light conditions for ion pumping by bacteriorhodopsin and/or halorhodopsin. The incoming signal is transferred across the plasma membrane by means of receptor-specific transducer proteins that bind tightly to their corresponding photoreceptors. To investigate the receptor/transducer interaction, advantage is taken of the observation that both SRI and SRII can function as proton pumps. SRI from Halobacterium salinarum, which triggers the positive phototaxis, the photophobic receptor SRII from Natronobacterium pharaonis (pSRII), as well as the mutant pSRII-F86D were expressed in Xenopus oocytes. Voltage-clamp studies confirm that SRI and pSRII function as light-driven, outwardly directed proton pumps with a much stronger voltage dependence than the ion pumps bacteriorhodopsin and halorhodopsin. Coexpression of SRI and pSRII-F86D with their corresponding transducers suppresses the proton transport, revealing a tight binding and specific interaction of the two proteins. These latter results may be exploited to further analyze the binding interaction of the photoreceptors with their downstream effectors.
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