ArticleProceedings of the National Academy of Sciences of the United States of America2001
Transcript leader regions of two Saccharomyces cerevisiae mRNAs contain internal ribosome entry sites that function in living cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 76 citations in OpenAlex.
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- yGPS-P: A Yeast-Based Peptidome Screen for Studying Quality Control-Associated Proteolysis.Biomolecules · 2023Article
- Synthetic polycistronic sequences in eukaryotes.Synthetic and systems biotechnology · 2021Review
- Screening for functional IRESes using α-complementation system of β-galactosidase inBiotechnology for biofuels · 2019Article
- The 5' Untranslated Region of themSphere · 2018Article
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- IRES-dependent translated genes in fungi: computational prediction, phylogenetic conservation and functional association.BMC genomics · 2015Article
- Role of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells.Molecular medicine reports · 2015Article
- Hydrogen peroxide sensing, signaling and regulation of transcription factors.Redox biology · 2014Review
- Synthetic RNA modules for fine-tuning gene expression levels in yeast by modulating RNase III activity.Nucleic acids research · 2011Article
- A synthetic library of RNA control modules for predictable tuning of gene expression in yeast.Molecular systems biology · 2011Article
- Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae.Yeast (Chichester, England) · 2010Article
- An internal ribosomal entry site mediates redox-sensitive translation of Nrf2.Nucleic acids research · 2010Article
- Translation initiation factors are not required for Dicistroviridae IRES function in vivo.RNA (New York, N.Y.) · 2009Article
- A small stem loop element directs internal initiation of the URE2 internal ribosome entry site in Saccharomyces cerevisiae.The Journal of biological chemistry · 2008Article
- Identification of putative regulatory upstream ORFs in the yeast genome using heuristics and evolutionary conservation.BMC bioinformatics · 2007Article
- Zuotin, a DnaJ molecular chaperone, stimulates cap-independent translation in yeast.Biochemical and biophysical research communications · 2006Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
In higher eukaryotes, translation of some mRNAs occurs by internal initiation. It is not known, however, whether this mechanism is used to initiate the translation of any yeast mRNAs. In this report, we identify naturally occurring nucleotide sequences that function as internal ribosome entry sites (IRESes) within the 5' leader sequences of Saccharomyces cerevisiae YAP1 and p150 mRNAs. When tested in the 5' untranslated regions of monocistronic reporter genes, both leader sequences enhanced translation efficiency in vegetatively growing yeast cells. Moreover, when tested in the intercistronic region of dicistronic mRNAs, both sequences were shown to contain IRESes that functioned in living cells. The activity of the p150 leader was much greater than that of the YAP1 leader. The second cistron was not expressed in control dicistronic constructs that lacked these sequences or contained the 5' leader sequence of the CLN3 mRNA in the intercistronic region. Further analyses of the p150 IRES revealed that it contained several nonoverlapping segments that were able independently to mediate internal initiation. These results suggested a modular composition for the p150 IRES that resembled the composition of IRESes contained within some cellular mRNAs of higher eukaryotes. Both YAP1 and p150 leaders contain several complementary sequence matches to yeast 18S rRNA. The findings are discussed in terms of our understanding of internal initiation in higher eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.