ArticleProceedings of the National Academy of Sciences of the United States of America2001
Ash1p is a site-specific DNA-binding protein that actively represses transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins.Microbial cell factories · 2024Article
- Profiling proteomic responses to hexokinase-II depletion in terpene-producingEngineering microbiology · 2023Article
- Structure of a SIN3-HDAC complex from budding yeast.Nature structural & molecular biology · 2023Article
- Genome-wide identification of the GATA transcription factor family and their expression patterns under temperature and salt stress in Aspergillus oryzae.AMB Express · 2021Article
- Ash1 and Tup1 dependent repression of the Saccharomyces cerevisiae HO promoter requires activator-dependent nucleosome eviction.PLoS genetics · 2020Article
- FACT and Ash1 promote long-range and bidirectional nucleosome eviction at the HO promoter.Nucleic acids research · 2020Article
- Multiple Negative Regulators Restrict Recruitment of the SWI/SNF Chromatin Remodeler to theGenetics · 2019Article
- Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression.PloS one · 2018Article
- Article
- The Roles of Puf6 and Loc1 in 60S Biogenesis Are Interdependent, and Both Are Required for Efficient Accommodation of Rpl43.The Journal of biological chemistry · 2016Article
- Creating memories of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Spatial regulation of translation through RNA localization.F1000 biology reports · 2012Article
- Review
- SCFCdc4 enables mating type switching in yeast by cyclin-dependent kinase-mediated elimination of the Ash1 transcriptional repressor.Molecular and cellular biology · 2011Article
- Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae.PloS one · 2010Article
- Daughter-specific transcription factors regulate cell size control in budding yeast.PLoS biology · 2009Article
- She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- Functional and physical interactions within the middle domain of the yeast mediator.Molecular genetics and genomics : MGG · 2006Article
- Daughter-specific repression of Saccharomyces cerevisiae HO: Ash1 is the commander.EMBO reports · 2004Review
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2 authors.
Funding
Abstract
ASH1 encodes a protein that is localized specifically to the daughter cell nucleus, where it has been proposed to repress transcription of the HO gene. Using Ash1p purified from baculovirus-infected insect cells, we have shown that Ash1p binds specific DNA sequences in the HO promoter. DNase I protection analyses showed that Ash1p recognizes a consensus sequence, YTGAT. Mutation of this consensus abolishes Ash1p DNA binding in vitro. We have shown that Ash1p requires an intact zinc-binding domain in its C terminus for repression of HO in vivo and that this domain may be involved in DNA binding. A heterologous DNA-binding domain fused to an N-terminal segment of Ash1p functions as an active repressor of transcription. Our studies indicate that Ash1p is a DNA-binding protein of the GATA family with a separable transcriptional repression domain.
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