ArticleGut2001
FAS engagement drives apoptosis of enterocytes of coeliac patients.
Article in Gut, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 83 citations in OpenAlex.
- Immunological indicators of coeliac disease activity are not altered by long-term oats challenge.Clinical and experimental immunology · 2013Trial
- Artificial Intelligence Analysis of Celiac Disease Using an Autoimmune Discovery Transcriptomic Panel Highlighted Pathogenic Genes including BTLA.Healthcare (Basel, Switzerland) · 2022Article
- Coeliac Disease Pathogenesis: The Uncertainties of a Well-Known Immune Mediated Disorder.Frontiers in immunology · 2020Review
- Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2.Nature communications · 2019Article
- Architectural and functional alterations of the small intestinal mucosa in classical Whipple's disease.Mucosal immunology · 2017Article
- Intestinal and Circulating MicroRNAs in Coeliac Disease.International journal of molecular sciences · 2017Review
- Celiac Disease: Role of the Epithelial Barrier.Cellular and molecular gastroenterology and hepatology · 2017Review
- Ex vivo culture of intestinal crypt organoids as a model system for assessing cell death induction in intestinal epithelial cells and enteropathy.Cell death & disease · 2014Article
- Article
- Gliadin activates HLA class I-restricted CD8+ T cells in celiac disease intestinal mucosa and induces the enterocyte apoptosis.Gastroenterology · 2008Article
- Growth factor-like activity of gliadin, an alimentary protein: implications for coeliac disease.Gut · 2007Article
- Serological and clinical comparison of children and adults with anti-endomysial antibodies.Journal of clinical immunology · 2007Article
- Coeliac disease: an update for pathologists.Journal of clinical pathology · 2006Review
- Immunohistochemical analysis of coeliac mucosa following ingestion of oats.Clinical and experimental immunology · 2006Article
- Article
- Cytokine production by intestinal intraepithelial lymphocyte subsets in celiac disease.Digestive diseases and sciences · 2005Article
- Association of FAS (TNFRSF6)-670 gene polymorphism with villous atrophy in coeliac disease.World journal of gastroenterology · 2004Article
- Celiac disease: a model autoimmune disease with gene therapy applications.Gene therapy · 2003Review
- Immunohistochemical study of the apoptotic mechanisms in the intestinal mucosa during children's coeliac disease.Virchows Archiv : an international journal of pathology · 2003Article
- Interleukin 18 and associated markers of T helper cell type 1 activity in coeliac disease.Gut · 2002Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundVillus atrophy is the most distinctive sign of untreated coeliac disease (CD) and epithelial apoptosis is considered to be involved in this stage of the coeliac lesion. The extent of villus atrophy is, however, not homogeneous and patients with patchy or mild lesions have been described.
aimsTo address: (a) the degree of "patchiness" in untreated CD patients; and (b) to clarify if apoptosis, and eventually which trigger drives it, causes epithelial damage. PATIENTS: Twenty of 40 untreated, 14 treated coeliac patients, and 15 controls received five or more multiple duodenal biopsies; the remaining 20 untreated CD patients had no more than three biopsies.
methodsAll biopsies were analysed to monitor the presence of a "flat" mucosa. Biopsies of 14 untreated, 10 treated coeliacs, and seven controls were cultured with or without gliadin. DNA fragmentation was studied by terminal deoxynucleotidyl transferase (TdT) mediated dUTP digoxigenin nick end labelling (TUNEL), and FAS and Ki67 expression by immunohistochemistry. Antiendomysium antibodies (EMA) were surveyed in biopsy culture supernatants.
resultsA pattern of patchy duodenal lesions was observed in all untreated CD patients biopsied up to five times. High enterocyte FAS expression, and a high number of TUNEL+ and Ki67+ enterocytes were detected in areas with villus atrophy but not in those with a normal morphology (p<0.001). Conversely, EMA in culture supernatants and signs of immunological activation were present in all untreated CD biopsies. In vitro gliadin challenge increased the number of TUNEL+ and Ki67+ enterocytes (p<0.001 v cultures with medium alone) only in "flat" biopsies. Neutralising anti-FAS monoclonal antibodies were found to control gliadin induced enterocyte apoptosis (p>0.01) while agonist anti-FAS monoclonal antibody increased it (p<0.001).
conclusionsPatchy lesions are observed in untreated CD mucosa and epithelial FAS engagement is a key trigger in driving villus atrophy in CD.
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