ArticleThe Biochemical journal2001
Characterization of a cDNA encoding a novel avian hypothalamic neuropeptide exerting an inhibitory effect on gonadotropin release.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 180 citations in OpenAlex.
- Review
- An Inhibitory Circuit From Brainstem to GnRH Neurons in Male Mice: A New Role for the RFRP Receptor.Endocrinology · 2021Article
- RF-amide related peptide-3 (RFRP-3): a novel neuroendocrine regulator of energy homeostasis, metabolism, and reproduction.Molecular biology reports · 2021Review
- Should I Lay or Should I Grow: Photoperiodic Versus Metabolic Cues in Chickens.Frontiers in physiology · 2020Review
- The Gonadotropin-Inhibitory Hormone: What We Know and What We Still Have to Learn From Fish.Frontiers in endocrinology · 2019Review
- Gonadotrophin-inhibitory hormone and its mammalian orthologue RFamide-related peptide-3: Discovery and functional implications for reproduction and stress.Journal of neuroendocrinology · 2018Review
- How to Contribute to the Progress of Neuroendocrinology: Discovery of GnIH and Progress of GnIH Research.Frontiers in endocrinology · 2018Review
- Comparative and Evolutionary Aspects of Gonadotropin-Inhibitory Hormone and FMRFamide-Like Peptide Systems.Frontiers in neuroscience · 2018Article
- Dual Actions of Mammalian and Piscine Gonadotropin-Inhibitory Hormones, RFamide-Related Peptides and LPXRFamide Peptides, in the Hypothalamic-Pituitary-Gonadal Axis.Frontiers in endocrinology · 2017Article
- A Journey through the Gonadotropin-Inhibitory Hormone System of Fish.Frontiers in endocrinology · 2017Review
- GnIH Control of Feeding and Reproductive Behaviors.Frontiers in endocrinology · 2016Review
- Reproductive Neuroendocrine Pathways of Social Behavior.Frontiers in endocrinology · 2016Review
- Seasonal control of gonadotropin-inhibitory hormone (GnIH) in birds and mammals.Frontiers in neuroendocrinology · 2015Review
- Dissecting the Roles of Gonadotropin-Inhibitory Hormone in Mammals: Studies Using Pharmacological Tools and Genetically Modified Mouse Models.Frontiers in endocrinology · 2015Review
- Contribution of GnIH Research to the Progress of Reproductive Neuroendocrinology.Frontiers in endocrinology · 2015Review
- Gonadotropin surge-inhibiting/attenuating factors: a review of current evidence, potential applications, and future directions for research.Molecular reproduction and development · 2015Review
- Review: evolution of GnIH and related peptides structure and function in the chordates.Frontiers in neuroscience · 2014Review
- Central and direct regulation of testicular activity by gonadotropin-inhibitory hormone and its receptor.Frontiers in endocrinology · 2014Review
- Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.PloS one · 2014Article
- The Evolution and Variety of RFamide-Type Neuropeptides: Insights from Deuterostomian Invertebrates.Frontiers in endocrinology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously isolated a novel dodecapeptide containing a C-terminal -Arg-Phe-NH(2) sequence, SIKPSAYLPLRF-NH(2) (RFamide peptide), from the quail brain. This quail RFamide peptide was shown to decrease gonadotropin release from the cultured anterior pituitary and to be located at least in the quail hypothalamo-hypophysial system. We therefore designated this RFamide peptide gonadotropin inhibitory hormone (GnIH). In the present study we characterized the GnIH cDNA from the quail brain by a combination of 3' and 5' rapid amplification of cDNA ends ('RACE'). The deduced GnIH precursor consisted of 173 amino acid residues, encoding one GnIH and two putative gene-related peptide (GnIH-RP-1 and GnIH-RP-2) sequences that included -LPXRF (X=L or Q) at their C-termini. All these peptide sequences were flanked by a glycine C-terminal amidation signal and a single basic amino acid on each end as an endoproteolytic site. Southern blotting analysis of reverse-transcriptase-mediated PCR products demonstrated a specific expression of the gene encoding GnIH in the diencephalon including the hypothalamus. Furthermore, mass spectrometric analyses detected the mass numbers for matured GnIH and GnIH-RP-2, revealing that both peptides are produced from the precursor in the diencephalon as an endogenous ligand. Taken together, these results lead to the conclusion that GnIH is a hypothalamic factor responsible for the negative regulation of gonadotropin secretion. Furthermore, the presence of a novel RFamide peptide family containing a C-terminal -LPXRF-NH(2) sequence has been revealed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.