ArticleThe Biochemical journal2001
Manipulation of the expression of regulatory genes of polyamine metabolism results in specific alterations of the cell-cycle progression.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 38 citations in OpenAlex.
- Chemoprevention of Colon Cancer by DFMO, Sulindac, and NO-Sulindac Administered Individually or in Combinations in F344 Rats.Cancers · 2023Article
- Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D.Scientific reports · 2019Article
- Targeting ornithine decarboxylase (ODC) inhibits esophageal squamous cell carcinoma progression.NPJ precision oncology · 2017Article
- The proapoptotic effect of traditional and novel nonsteroidal anti-inflammatory drugs in mammalian and yeast cells.Oxidative medicine and cellular longevity · 2013Review
- Spermidine promotes human hair growth and is a novel modulator of human epithelial stem cell functions.PloS one · 2011Article
- A review of gene-drug interactions for nonsteroidal anti-inflammatory drug use in preventing colorectal neoplasia.The pharmacogenomics journal · 2008Review
- Induction of spermidine/spermine N1-acetyltransferase (SSAT) by aspirin in Caco-2 colon cancer cells.The Biochemical journal · 2006Article
- A perspective of polyamine metabolism.The Biochemical journal · 2003Review
- Transcriptomic classification of antitumor agents: application to the analysis of the antitumoral effect of SR31747A.Gene expression · 2003Article
- Absolute requirement of spermidine for growth and cell cycle progression of fission yeast (Schizosaccharomyces pombe).Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We have previously reported that cyclical phases of accumulation and depletion of polyamines occur during cell-cycle progression. Regulatory ornithine decarboxylase (ODC) catalyses the first step of polyamine biosynthesis. Ornithine decarboxylase antizyme (OAZ), induced by high polyamine levels, inhibits ODC activity and prevents extracellular polyamine uptake. Spermidine/spermine N1-acetyltransferase (SSAT) regulates the polyamine degradation/excretion pathway. Here we show that 24 h transient transfection of immortalized human prostatic epithelial cells (PNT1A and PNT2) with antisense ODC RNA or OAZ cDNA, or both, while effectively causing marked decreases of ODC activity and polyamine (especially putrescine) concentrations, resulted in accumulation of cells in the S phase of the cell cycle. Transfection with SSAT cDNA led to more pronounced decreases in spermidine and spermine levels and resulted in accumulation of cells in the G2/M phases. Transfection with all three constructs together produced maximal depletion of all polyamines, accompanied by accumulation of PNT1A cells in the S phase and PNT2 cells in the G0/G1 and G2/M phases. Accumulation of PNT1A cells in the S phase progressively increased at 15, 18 and 24 h of transfection with antisense ODC and/or OAZ cDNA. At 24 h, the DNA content was always reduced, as a possible outcome of altered chromosome condensation. A direct link between polyamine metabolism, cell proliferation and chromatin structure is thus proposed.
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