ArticleThe Biochemical journal2001
Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Stem cell-derived small extracellular vesicles containing miR-27b-3p attenuated osteoarthritis through inhibition of leukaemia inhibitory factor.Fundamental research · 2025Article
- Review
- CD13/Aminopeptidase N Is a Potential Therapeutic Target for Inflammatory Disorders.Journal of immunology (Baltimore, Md. : 1950) · 2020Review
- Alzheimer's Disease: Mechanism and Approach to Cell Therapy.International journal of molecular sciences · 2015Review
- Proteomic analysis of urine exosomes reveals renal tubule response to leptospiral colonization in experimentally infected rats.PLoS neglected tropical diseases · 2015Article
- Lipid rafts and Alzheimer's disease: protein-lipid interactions and perturbation of signaling.Frontiers in physiology · 2012Article
- Inhibitory effect of anti-aminopeptidase N/CD13 antibodies on fibroblast migration.Molecular and cellular biochemistry · 2010Article
- Renin-angiotensin system in human coronavirus pathogenesis.Future virology · 2010Review
- Suppression of human prostate tumor growth by a unique prostate-specific monoclonal antibody F77 targeting a glycolipid marker.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Caveolae in fibroblast-like synoviocytes: static structures associated with vimentin-based intermediate filaments.Histochemistry and cell biology · 2009Article
- The moonlighting enzyme CD13: old and new functions to target.Trends in molecular medicine · 2008Review
- Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted.Journal of virology · 2007Article
- Article
- T-cell activation via CD26 and caveolin-1 in rheumatoid synovium.Modern rheumatology · 2006Review
- Regulation of aminopeptidase N (EC 3.4.11.2; APN; CD13) by interferon-gamma on the HL-60 cell line.Life sciences · 2005Article
- Dipeptidyl peptidase IV activity and/or structure homologs: contributing factors in the pathogenesis of rheumatoid arthritis?Arthritis research & therapy · 2005Review
- CD26 up-regulates expression of CD86 on antigen-presenting cells by means of caveolin-1.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae.Journal of virology · 2004Article
- Cell-surface peptidases.International review of cytology · 2004Review
- Deep-apical tubules: dynamic lipid-raft microdomains in the brush-border region of enterocytes.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane peptidases play important roles in cell activation, proliferation and communication. Human fibroblast-like synoviocytes express considerable amounts of aminopeptidase N/CD13, dipeptidyl peptidase IV/CD26, and neprilysin/CD10, transmembrane proteins previously proposed to be involved in the regulation of intra-articular levels of neuropeptides and chemotactic mediators as well as in adhesion and cell-cell interactions. Here, we report these peptidases in synoviocytes to be localized predominantly in glycolipid- and cholesterol-rich membrane microdomains known as 'rafts'. At the ultrastructural level, aminopeptidase N/CD13 and dipeptidyl peptidase IV/CD26 were found in caveolae, in particular in intracellular yet surface-connected vesicle-like structures and 'rosettes' made up of several caveolae. In addition, clusters of peptidases were seen at the cell surface in flat patches ranging in size from about 60 to 160 nm. Cholesterol depletion of synoviocytes by methyl-beta-cyclodextrin disrupted >90% of the caveolae and reduced the raft localization of aminopeptidase N/CD13 without affecting Ala-p-nitroanilide-cleaving activity of confluent cell cultures. In co-culture experiments with T-lymphocytes, cholesterol depletion of synoviocytes greatly reduced their capability to induce an early lymphocytic expression of aminopeptidase N/CD13. We propose caveolae/rafts to be peptidase-rich 'hot-spot' regions of the synoviocyte plasma membrane required for functional cell-cell interactions with lymphocytes. The peptidases may act in concert with other types of proteins such as receptors and signal transducers localized in these specialized membrane domains.
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