ArticleThe Biochemical journal2001
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Thymic epithelial cells require lipid kinase Vps34 for CD4 but not CD8 T cell selection.The Journal of experimental medicine · 2022Article
- Class III PI3K Biology.Current topics in microbiology and immunology · 2022Article
- BECN1-dependent CASP2 incomplete autophagy induction by binding to rabies virus phosphoprotein.Autophagy · 2017Article
- Autophagy and mTORC1 regulate the stochastic phase of somatic cell reprogramming.Nature cell biology · 2015Article
- PI(5)P regulates autophagosome biogenesis.Molecular cell · 2015Article
- Insulin-dependent apolipoprotein B degradation is mediated by autophagy and involves class I and class III phosphatidylinositide 3-kinases.Biochemical and biophysical research communications · 2013Article
- Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway.The Journal of cell biology · 2011Article
- The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila.The Journal of cell biology · 2008Article
- PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or sorting of procathepsin D by regulating multivesicular body morphogenesis.Molecular biology of the cell · 2003Article
- Phagosome maturation: a few bugs in the system.The Journal of membrane biology · 2003Review
- Mycobacterium tuberculosis glycosylated phosphatidylinositol causes phagosome maturation arrest.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Role of LAMP-2 in lysosome biogenesis and autophagy.Molecular biology of the cell · 2002Article
- Membrane transport in Caenorhabditis elegans: an essential role for VPS34 at the nuclear membrane.The EMBO journal · 2002Article
- Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid kinases and their phosphorylated products are important regulators of many cellular processes, including intracellular membrane traffic. The best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized carboxypeptidase Y to the yeast vacuole. A probable mammalian Vps34p orthologue has been previously identified, but its function in the trafficking of lysosomal enzymes has not been resolved. To investigate the possible role(s) of mammalian Vps34p in protein targeting to lysosomes, we have cloned the rat orthologue and overexpressed a kinase-deficient mutant in HeLa cells. Expression of the mutant protein inhibited both maturation of procathepsin D and basal secretion of the precursor. In contrast wortmannin, which also inhibited maturation, caused hypersecretion of the precursor. We propose that mammalian Vps34p plays a direct role in targeting lysosomal enzyme precursors to the endocytic pathway in an analogous fashion to its role in the fusion of early endocytic vesicles with endosomes. We further suggest that inhibition of a wortmannin-sensitive enzyme, other than mammalian Vps34p, is responsible for the failure to recycle unoccupied mannose 6-phosphate receptors to the trans-Golgi network, and consequent hypersecretion of lysosomal enzyme precursors observed in the presence of this drug.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.